Type of Document Dissertation Author Melnyk, Jason Alexander Author's Email Address email@example.com URN etd-02042009-200044 Title Contractile Performance and Energy Utilization of Skeletal Muscle; Creatine Kinase and Acto-myosin ATPase Degree PhD Department Human Nutrition, Foods, and Exercise Advisory Committee
Advisor Name Title Williams, Jay H. Committee Chair Davy, Kevin P. Committee Member Moore, David Michael Committee Member Newton, William E. Committee Member Rankin, Janet L. Walberg Committee Member Keywords
- contractile performance
- muscle energetics
Date of Defense 2009-01-23 Availability unrestricted AbstractCreatine kinase (CK) primarily serves as an energy buffer assisting in regulating ATP homeostasis through synthesis of ATP from ADP and phosphocreatine (PCr). This enzyme is bound in the sarcomere near sites of ATP consumption via acto-myosin ATPase (A•M•ATPase) and research in cardiac muscle has found that PCr can alter contractile performance (maximal isometric force and Ca2+ sensitivity). Based on this evidence, CK and A•M•ATPase may be coupled in skeletal muscle. Therefore the purpose of this investigation was to determine the influence of the CK system on contractile performance and energy utilization in skeletal muscle.
When skinned fibers (membrane removed) were provided a limited supply of [ATP] (0.1 mM), this resulted in increased Ca2+ sensitivity. The addition of PCr to low ATP solutions restored Ca2+ sensitivity and allowed normal isometric force generation across a range of [Ca2+] via ATP synthesis by CK. This was also possible with only CK reaction substrates (ADP, PCr) in the absence of ATP. Based on these findings, endogenous CK activity in glycerol skinned skeletal muscle fibers is sufficient to permit normal function of the contractile apparatus.
Energy utilization was studied by indirect assessment of ADP production. Decreased net ADP production as measured by NADH fluorescence revealed endogenous CK was able to convert ADP produced by A•M•ATPase to ATP in skeletal muscle across a range of both [Ca2+] and [ATP]. This was confirmed directly via high-performance liquid chromatography measurements of ATP and ADP by showing that skinned skeletal muscle bundles have sufficient endogenous CK activity to produce ATP from substrates (ADP, PCr) and the ability to maintain low [ADP] in the presence of PCr.
This study adds to the evidence for specific compartmentation of CK near sites of ATP utilization and contributes to the body of knowledge on contractile performance in skinned skeletal muscle fibers. By showing how changing demands on skeletal muscle (through increased Ca2+) alters force production and Ca2+ sensitivity, these findings lend support for the importance of endogenous CK as a pathway of ATP regeneration in skeletal muscle.
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