Type of Document	Master's Thesis
Author	Bennett, Lisa Tracy
URN	etd-02062013-040043
Title	Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
Degree	Master of Science
Department	Anaerobic Microbiology
Advisory Committee	Advisor Name Title Dean, Dennis R. Committee Chair Chen, Jiann-Shin Committee Member Johnson, John L. Committee Member Larson, Timothy J. Committee Member Sitz, Thomas O. Committee Member
Keywords	Klebsiella pneumoniae
Date of Defense	1989-12-15
Availability	restricted
Abstract Nitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from Azotobacter vinelandii were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in A vinelandii flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing conditions nifF gene expression is stimulated.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access  LD5655.V855_1989.B466.pdf 14.05 Mb 01:05:02 00:33:27 00:29:16 00:14:38 00:01:14 next to an author's name indicates that all files or directories associated with their ETD are accessible from the Virginia Tech campus network only.

If you have questions or technical problems, please Contact DLA.