Title page for ETD etd-02092005-233929


Type of Document Master's Thesis
Author Shah, Kinjalkumar K
URN etd-02092005-233929
Title Design, Synthesis and Characterization of Heme-proteins: Developing Potential Catalysts for Bio-remediation
Degree Master of Science
Department Biological Systems Engineering
Advisory Committee
Advisor Name Title
Zhang, Chenming Mike Committee Chair
Gillespie, Joel Committee Co-Chair
Bevan, David R. Committee Member
Cundiff, John S. Committee Member
Keywords
  • Peroxidase activity
  • Cytochrome b562
  • Protein design
  • Hemeprotein
  • Solid phase peptide synthesis
  • Four-helix bundle protein
Date of Defense 2004-12-16
Availability unrestricted
Abstract
The next generation of toxic chemicals and hazardous wastes from sophisticated chemical industries will demand the environmental agencies to employ biological methods over the conventional physical and chemical remediation methods. Over the past decade, natural metallo-enzymes have been identified to degrade some of the major chemical contaminants through electron transfer pathways. However, these natural enzymes are less stable in organic solvents and they are not effective for the degradation of toxic compounds such as polychlorinated biphenyls or dioxins. This thesis explores the use of protein design approaches to produce chemically and molecularly modified enzymes, which are highly stable, possess little substrate specificity, and have higher activity than the natural enzymes. The experiments presented in this thesis make use of solid phase synthesis and site-directed mutagenesis for the synthesis and production of these enzymes and popular chromatographic techniques for their purification. The partial characterization of these proteins revealed the essential structural features of these proteins, and their catalytic activity was demonstrated by the use of peroxidase assays.
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