Title page for ETD etd-02232006-012016


Type of Document Dissertation
Author Barthet, Michelle Marie
Author's Email Address mbarthet@vt.edu
URN etd-02232006-012016
Title Expression and Function of the Chloroplast-encoded Gene matK.
Degree PhD
Department Biological Sciences
Advisory Committee
Advisor Name Title
Hilu, Khidir W. Committee Chair
Beers, Eric P. Committee Member
Gillaspy, Glenda E. Committee Member
Sible, Jill C. Committee Member
Walker, Richard A. Committee Member
Keywords
  • chloroplast
  • fast-evolving
  • maturase
  • MatK
  • Orchidaceae
Date of Defense 2006-02-09
Availability unrestricted
Abstract
The chloroplast matK gene has been identified as a rapidly evolving gene at nucleotide and corresponding amino acid levels. The high number of nucleotide substitutions and length mutations in matK has provided a strong phylogenetic signal for resolving plant phylogenies at various taxonomic levels. However, these same features have raised questions as to whether matK produces a functional protein product. matK is the only proposed chloroplast-encoded group II intron maturase. There are 15 genes in the chloroplast that would require a maturase for RNA splicing. Six of these genes have introns that are not excised by a nuclear imported maturase, leaving MatK as the only candidate for processing introns in these genes. Very little research has been conducted concerning the expression and function of this important gene and its protein product. It has become crucial to understand matK expression in light of its significance in RNA processing and plant systematics. In this study, we examined the expression, function and evolution of MatK using a combination of molecular and genetic methods. Our findings indicate that matK RNA and protein is expressed in a variety of plant species, and expression of MatK protein is regulated by development. In addition, matK RNA levels are affected by light. Furthermore, genetic analysis has revealed that although MatK has a high rate of amino acid substitution, these substitutions are not random but are constrained to maintain overall chemical structure and stability in this protein. We have also identified an alternate start codon for matK in some plant species that buffers indels (insertions and deletions) in the open reading frame (ORF) that are not in multiples of three in the gene sequence. Usually, indels not in multiples of three result in frame shifts that destroy the reading frame. Our results indicate that an out-of-frame matK start codon in some orchids compensates for these otherwise deleterious indels. This research represents the first in-depth analysis of matK gene expression and contributes to several fields of biology including plant systematics, genetics and gene expression.
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