Title page for ETD etd-02262007-095956


Type of Document Dissertation
Author Angeloni, Stephen V.
URN etd-02262007-095956
Title Characterization of the nifUHD cluster and a new myoglobin-like gene from Nostoc commune UTEX 584
Degree PhD
Department Biochemistry and Nutrition
Advisory Committee
Advisor Name Title
Potts, Malcolm Committee Chair
Hess, John L. Committee Member
Keenan, Thomas W. Committee Member
Lacy, George H. Committee Member
Larson, Timothy J. Committee Member
Keywords
  • Cyanoglobin
  • Nitrogen-fixing microorganisms
  • Myoglobin
Date of Defense 1992-03-04
Availability restricted
Abstract

Sequence analysis of the entire 3.5 kb HindIII genomic DNA fragment previously isolated from Nostoc commune UTEX 584 (Defrancesco and Potts 1988), determined the exact locations of the nifU, nifH, and nifD genes and identified two potential stem loop structures, a direct repeat, and an ORF that codes for a protein with a predicted amino acid sequence similar to that of myoglobin from Paramecium caudatum.

The N. commune UTEX 584 myoglobin-like protein has a predicted length of 118 amino acids and molecular mass of 12,906 Da. A PCR copy of the gene (glbN) was cloned for overexpression of the protein. The recombinant protein was purified and used for spectral analysis and for the production of polyclonal antisera.

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