Type of Document	Dissertation
Author	Angeloni, Stephen V.
URN	etd-02262007-095956
Title	Characterization of the nifUHD cluster and a new myoglobin-like gene from Nostoc commune UTEX 584
Degree	PhD
Department	Biochemistry and Nutrition
Advisory Committee	Advisor Name Title Potts, Malcolm Committee Chair Hess, John L. Committee Member Keenan, Thomas W. Committee Member Lacy, George H. Committee Member Larson, Timothy J. Committee Member
Keywords	Cyanoglobin Nitrogen-fixing microorganisms Myoglobin
Date of Defense	1992-03-04
Availability	restricted
Abstract Sequence analysis of the entire 3.5 kb HindIII genomic DNA fragment previously isolated from Nostoc commune UTEX 584 (Defrancesco and Potts 1988), determined the exact locations of the nifU, nifH, and nifD genes and identified two potential stem loop structures, a direct repeat, and an ORF that codes for a protein with a predicted amino acid sequence similar to that of myoglobin from Paramecium caudatum. The N. commune UTEX 584 myoglobin-like protein has a predicted length of 118 amino acids and molecular mass of 12,906 Da. A PCR copy of the gene (glbN) was cloned for overexpression of the protein. The recombinant protein was purified and used for spectral analysis and for the production of polyclonal antisera.
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