Type of Document Master's Thesis Author Sedano, Rodolfo Canseco URN etd-03122013-040259 Title Effect of immunoglobulins on early bovine embryo development in vitro Degree Master of Science Department Dairy Science Advisory Committee
Advisor Name Title Gwazdauskas, Francis C. Committee Chair Barnes, Michael A. Committee Member Vinson, William E. Committee Member White, John M. Committee Member Keywords
- Veterinary embryology
Date of Defense 1985-11-05 Availability restricted AbstractBovine morulae (day 6: n=257) were obtained to evaluate
the [effect of immunoglobulins (Ig) on early bovine embryo
development in vitro. Fifty—four cows superovulations were
conducted in 36 cows with follicle stimulating hormone.
Embryos were collected by non-surgical procedures and
morphologically evaluated and randomly assigned to culture.
Embryos were cultured in Ham’s F-10 containing 10%
(6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma .
globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml)
bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS
plus 1% 1gM. Embryos were cultured to the hatched blastocyst
lstage or degeneration and evaluated at 12 h intervals.
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