Type of Document	Master's Thesis
Author	Sedano, Rodolfo Canseco
URN	etd-03122013-040259
Title	Effect of immunoglobulins on early bovine embryo development in vitro
Degree	Master of Science
Department	Dairy Science
Advisory Committee	Advisor Name Title Gwazdauskas, Francis C. Committee Chair Barnes, Michael A. Committee Member Vinson, William E. Committee Member White, John M. Committee Member
Keywords	Veterinary embryology
Date of Defense	1985-11-05
Availability	restricted
Abstract Bovine morulae (day 6: n=257) were obtained to evaluate the [effect of immunoglobulins (Ig) on early bovine embryo development in vitro. Fifty—four cows superovulations were conducted in 36 cows with follicle stimulating hormone. Embryos were collected by non-surgical procedures and morphologically evaluated and randomly assigned to culture. Embryos were cultured in Ham’s F-10 containing 10% (6.4 mg/ml) steer serum (SS), 1% (.64 mg/ml) bovine gamma . globulins (GG), 1% (.64 mg/ml) bovine IgG, 1% (.64 mg/ml) bovine 1gM, 10% SS plus 1% GG, 10% SS plus 1% 1gG, or 10% SS plus 1% 1gM. Embryos were cultured to the hatched blastocyst lstage or degeneration and evaluated at 12 h intervals.
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