Type of Document Master's Thesis Author Sink, Carolyn Author's Email Address firstname.lastname@example.org URN etd-04042002-070623 Title Canine Platelet Concentrates: An In Vitro Study to Effectively Provide a Source of Functional Platelets Degree Master of Science Department Veterinary Medical Sciences Advisory Committee
Advisor Name Title Feldman, Bernard F. Committee Chair Monroe, William Edward Committee Member Panciera, David L. Committee Member Keywords
- canine platelet concentrate
- storage lesion
- blood component
Date of Defense 2002-03-25 Availability unrestricted AbstractThis study monitored the storage lesion of 15 units of canine platelet concentrates harvested by differential centrifugation. Canine platelet concentrates were stored at 20-24° C in a platelet rotator for a total of 9 days; the storage lesion of three second generation platelet storage containers was compared. The battery of in vitro tests used to monitor the storage lesion were selected from previous studies performed with human platelet concentrates separated by differential centrifugation. Based on these tests, canine platelet concentrates exhibited a storage lesion similar to human platelet concentrates. Metabolic analytes demonstrated decreasing pH, carbon dioxide, bicarbonate and glucose concentrations concurrent with increasing oxygen and lactate dehydrogenase activity over the 9-day period. Platelet structural changes were monitored by mean platelet volume, which began to increase on Day-5. Platelet function appeared to be compromised, as indicated by aggregation studies using collagen and adenosine diphosphate as agonists. Product sterility was maintained.
There was no consensus of data supporting superior performance of one platelet storage container. This study indicates that canine platelet concentrates may be harvested by differential centrifugation of whole blood. In vitro studies utilizing three second-generation platelet storage bags support a previous study and concurs that canine platelet concentrates stored at 20-24° C using continuous agitation are viable for at least 5 days.
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