Title page for ETD etd-04072010-020253
|Type of Document
||Maciorowski, Anthony F.
||The development and assessment of an automated biological monitoring system using crayfish locomotor activity to detect toxic stress.
|Benfield, Ernest Fredrick
|Cairns, John Jr.
|Cochran, Donald G.
|Dickson, Kenneth L.
|Hendricks, Albert C.
|Patterson, Robert A.
|Date of Defense
In-plant biomonitors, controlled by dedicated microcomputers, may
ultimately provide detection of developing toxicity and hazardous
spills in industrial effluents rapidly enough to prevent serious damage
to receiving waters. A computer automated system was developed to continuously
monitor spontaneous locomotor activity of eight crayfish.
Electric potentials generated by muscular movements of untethered crayfish
were detected by external electrodes, amplified, digitized, and
analyzed by a hybrid computer network. A microcomputer evaluated the
number of peaks produced in the waveforms of eight individual C. acuminatus
for successive one-hour intervals. The resultant counts were directly
related to locomotor activity. The effectiveness of the crayfish biomonitor
in detecting simulated spills of cadmium in the laboratory was
determined by 7 experiments with 56 crayfish. Results indicated that
crayfish responded to 2.5 and 0.5 mg Cd++/l with increased activity,
which was detected by the crayfish biomonitor two hours after exposure.
A simulated spill of O.1 mgCd++/l was detected by the biomonitor within
113 hours. These results support the assumption that the crayfish activity
monitor may be used as a potential tool for water quality management.
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