Type of Document Master's Thesis Author Porter, Caroline Yancey Author's Email Address firstname.lastname@example.org URN etd-04102001-180241 Title Inheritance of the Gene(s) Controlling Leaflet Shape in Soybean Degree Master of Science Department Crop and Soil Environmental Sciences Advisory Committee
Advisor Name Title Buss, Glenn R. Committee Chair Griffey, Carl A. Committee Member Veilleux, Richard E. Committee Member Keywords
- Glycine soja
- Glycine max
- lanceolate leaf shape
Date of Defense 2000-12-15 Availability unrestricted AbstractMany soybean [Glycine max (L.) Merrill] cultivars have narrow leaflet shape but it is not known if all of these lines derive this trait from the ln gene or another locus. This project was conducted to determine the inheritance of the narrow leaflet trait in several soybean genotypes and wild [Glycine soja Sieb. et Zucc.] accessions, and also to determine the allelism of the genes for this trait in the selected lines. The parents, F1, F2 and F2:3 generations were grown at Kentland Research Farm near Blacksburg, VA or in the greenhouse.
The F2 and F2:3 generations (where available) were observed for segregation in leaflet shape. The populations were scored as having either broad or narrow leaflets using visual classification and leaf measurements when necessary. 'Camp' was crossed with broad leaflet parent 'Essex' to study the inheritance of the narrow leaflet trait in Camp. Observation of the F2 and F2:3 generations lead to the conclusion that a single recessive gene controls leaflet shape in Camp. Narrow leaf parents 'SRF 400' and Camp were crossed with lines having the ln gene (T41, S56, and D64-4731). None of the crosses among Camp, T41, SRF 400, S56 and D64-4731 segregated for leaflet shape in the F2 generation leading to the conclusion that they all have the ln allele at the same locus controlling lanceolate leaflet shape. T313, a line containing a gene for narrow rugose leaflets (lnr), was crossed with Camp to study allelism between the lnr and ln genes.
Segregation for leaflet shape was observed in the F2 and F2:3 generations allowing the conclusion that the lnr gene controlling the narrow rugose leaflet trait in T313 is at a locus independent from the ln gene. A deficiency of narrow rugose plants was observed in all of the populations with T313 as a parent, and was theorized as being caused by selection against lnr gametes. After adjustment for the lnr deficiency, the F2 data appeared to fit a 9 broad : 3 narrow : 4 narrow rugose ratio. Three G. soja lines were crossed to broad and narrow leaflet parents and the F2 generations were examined to determine the inheritance of the very narrow leaf phenotype. The results indicate that there are one or two recessive genes controlling narrow leaflet shape in the G. soja accessions, which are not allelic to the ln gene. Since these populations were not advanced to the F3 generation, definite conclusions cannot be drawn about the genetics of the very narrow leaf phenotype.
Filename Size Approximate Download Time (Hours:Minutes:Seconds)
28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access Thesis.PDF 986.41 Kb 00:04:34 00:02:20 00:02:03 00:01:01 00:00:05
If you have questions or technical problems, please Contact DLA.