Type of Document Master's Thesis Author Becvarova, Iveta Author's Email Address email@example.com URN etd-04132006-103946 Title Peroxidative protection of parenteral admixture by d-á-tocopherol and its effect on oxidative status of obese cats Degree Master of Science Department Biomedical and Veterinary Sciences Advisory Committee
Advisor Name Title Saker, Korinn E. Committee Chair Swecker, William S. Jr. Committee Member Troy, Gregory C. Committee Member Keywords
- lipid peroxidation
- vitamin E
- parenteral nutrition
Date of Defense 2006-03-30 Availability unrestricted AbstractHigh lipid : low dextrose (HL:LD) parenteral admixture (PA) is high in polyunsaturated fatty acids (PUFA) that are sensitive to peroxidation. This study evaluated the antioxidative effect of vitamin E in both HL:LD PA and in obese cats given HL:LD PA. Natural d-á-tocopherol (Vital E-300) was added to HL:LD PA at seven concentrations (8, 12, 16, 24, 32, 48, or 64 IU/g of lipid). PA were exposed to fluorescent light for 24 hours at room temperature. Hydroperoxides were measured at baseline and 24 hours hang time. Significantly lower hydroperoxide concentrations were found with > 24 IU/g of lipid at baseline (P < 0.01). A higher d-á-tocopherol concentration was required (> 48 IU/g lipid) to lower hydroperoxides at 24 hours (P < 0.0001). HL:LD PA with 40 IU/g lipid/day d-á-tocopherol was delivered intravenously to obese cats (PA Toc+) over 48 hours. Control cats (PA Toc-) received HL:LD PA without a d-á-tocopherol supplementation. Oxidative status of cats was evaluated at baseline and 24, 48, and 96 hours. Cats in both groups exhibited an increase in MDA concentration (time effect; P < 0.0001). WBC-tGSH and WBC-GPx did not change in either group of cats. RBC-tGSH and RBC-GPx changed over time (time effects; P = 0.0005; P = 0.0016, respectively) with the PA Toc+ cats exhibiting a higher RBC-tGSH concentration (treatment x time interaction; P = 0.012). Serum á- and ã-tocopherol concentrations increased in PA Toc+ cats (treatment effect; P < 0.0001). These findings suggest that d-á-tocopherol significantly alters oxidative status in vivo.
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