Title page for ETD etd-04222003-171204


Type of Document Master's Thesis
Author Szeles, Cheryl Lynne
URN etd-04222003-171204
Title Determining Sources of Fecal Contamination in Two Rivers of Northumberland County, Virginia
Degree Master of Science
Department Crop and Soil Environmental Sciences
Advisory Committee
Advisor Name Title
Hagedorn, Charles III Committee Chair
Eick, Matthew J. Committee Member
Reneau, Raymond B. Jr. Committee Member
Keywords
  • Escherichia coli
  • bacterial source tracking
  • watersheds
  • shellfish
Date of Defense 2003-04-09
Availability restricted
Abstract
DETERMINING SOURCES OF FECAL CONTAMINATION IN TWO RIVERS OF NORTHUMBERLAND COUNTY, VIRGINIA By Cheryl Lynne Szeles Dr. Charles Hagedorn III, Chairman Crop and Soil Environmental Sciences (ABSTRACT)

The goal of monitoring the water quality of shellfish beds is to provide protection against transmission of water-borne infectious diseases. The Coan River and the Little Wicomico River contain shellfish beds that are closed to harvest due to contamination with fecal bacteria. These two rivers are located in Northumberland County, Virginia, and empty into the lower Potomac River and the Chesapeake Bay.

Bacterial source tracking (antibiotic resistance analysis of Eschericia coli) was used to determine the sources of fecal contamination that have caused shellfish harvest closures in these two rivers.

A total of 1,248 Eschericia coli isolates were collected from known sources to build a regional library for the rivers. The Virginia Department of Shellfish Sanitation (DSS) and project cooperators collected known source samples from August 2001 to September 2002.

The Average Rate of Correct Classification for the known source library was 71.9%, with a total of 930 isolates correctly classified. The categories (and rates of correct classification) were Birds (84.7%), Humans (74.8%), Livestock (72.4%), Pets (62.0%), and Wildlife (65.7%). The library was used to identify the sources of Eschericia coli isolated from DSS sampling stations along the Coan and Little Wicomico Rivers from August 2001 to September 2002. Some stations contained a substantial human signature, while wildlife and birds are also major contributors. The results will be used to decide the necessary changes that need to be addressed if the shellfish harvesting beds are to be reopened. ii iii

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[VT] CLSETDAbstractB.pdf 64.54 Kb 00:00:17 00:00:09 00:00:08 00:00:04 < 00:00:01
[VT] CLSETDAcknowledgements2.pdf 54.11 Kb 00:00:15 00:00:07 00:00:06 00:00:03 < 00:00:01
[VT] CLSETDAppendix12.pdf 26.23 Kb 00:00:07 00:00:03 00:00:03 00:00:01 < 00:00:01
[VT] CLSETDAppendix13.pdf 221.85 Kb 00:01:01 00:00:31 00:00:27 00:00:13 00:00:01
[VT] CLSETDDedication1.pdf 26.04 Kb 00:00:07 00:00:03 00:00:03 00:00:01 < 00:00:01
[VT] CLSETDIntroduction6.pdf 63.55 Kb 00:00:17 00:00:09 00:00:07 00:00:03 < 00:00:01
[VT] CLSETDListofFigures5.pdf 40.66 Kb 00:00:11 00:00:05 00:00:05 00:00:02 < 00:00:01
[VT] CLSETDListofTables4.pdf 76.28 Kb 00:00:21 00:00:10 00:00:09 00:00:04 < 00:00:01
[VT] CLSETDLiteratureCited11.pdf 93.97 Kb 00:00:26 00:00:13 00:00:11 00:00:05 < 00:00:01
[VT] CLSETDMaterials&Methods8.pdf 233.96 Kb 00:01:04 00:00:33 00:00:29 00:00:14 00:00:01
[VT] CLSETDResults&Discussion9.pdf 252.92 Kb 00:01:10 00:00:36 00:00:31 00:00:15 00:00:01
[VT] CLSETDReviewofLiterature7.pdf 114.04 Kb 00:00:31 00:00:16 00:00:14 00:00:07 < 00:00:01
[VT] CLSETDSummary&Conclusions10.pdf 67.54 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01
[VT] CLSETDTableofContents3.pdf 58.70 Kb 00:00:16 00:00:08 00:00:07 00:00:03 < 00:00:01
[VT] CLSETDTitlePageA.pdf 65.62 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01
[VT] CLSETDVita14.pdf 45.70 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01
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