Title page for ETD etd-04252008-192512


Type of Document Master's Thesis
Author Lei, Guangyin
Author's Email Address guangyin@vt.edu
URN etd-04252008-192512
Title Synthesis of Nano-Silver Colloids and Their Anti-Microbial Effects
Degree Master of Science
Department Materials Science and Engineering
Advisory Committee
Advisor Name Title
Lu, Guo-Quan Committee Chair
Meehan, Kathleen Committee Co-Chair
Yang, Zhaomin Committee Member
Keywords
  • Bactericide
  • Escherichia coli
  • Staphylococcus aureus.
  • Silver nanoparticles
Date of Defense 2007-11-08
Availability unrestricted
Abstract
The antimicrobial effects of silver nanoparticles were studied. Silver nanoparticles were synthesized through wet chemistry method, and were dispersed in aqueous suspension. With nanoscale silica particles served as heterogeneous nucleation sites, silver nanoparticles were formed anchoring on the silica surface. Suspensions were found to be stable at high silver concentrations as well as over a broad pH range. By varying the processing conditions, diameter of the silver nanoparticles could be controlled between ~2 nm to ~25 nm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to reveal the formation and the corresponding morphology of the silver-silica coupling nanoparticles. Ultra-violet visible (UV-vis) scanning spectrophotometer was used to detecting the distinct absorption spectrum of silver nanoparticles. The antimicrobial activities of these silver-silica coupling nanoparticles were investigated. E. coli and S aureus were used as representatives of Gram-negative and Gram-positive bacteria, respectively. Bacteriological tests showed either bacterial growth inhibition or cell death occurred, corresponding to different concentrations of silver nanoparticles and the type of bacteria that were testing on. Fluorescent microscopic images were also provided to confirm the bacterial viability after several hours’ treatment with silver nanoparticles.
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