Type of Document	Dissertation
Author	Lai, Kun-Nan
URN	etd-05042006-164528
Title	Cloning and expression of cambialistic Bacteroides fragilis superoxide dismutase gene
Degree	PhD
Department	Biochemistry and Nutrition
Advisory Committee	Advisor Name Title Gregory, Eugene M. Committee Chair Hess, John L. Committee Chair Bevan, David R. Committee Member Johnson, John L. Committee Member Larson, Timothy J. Committee Member
Keywords	Gene expression Superoxide dismutase Molecular cloning
Date of Defense	1992-09-15
Availability	restricted
Abstract A gene coding for the cambialistic superoxide dismutase (SOD) was isolated from a LambdaGEM-ll genomic library of Bacteroides fragilis. In order to generate a complete genomic library, B. fragilis genomic DNA was partially digested with the restriction endonuclease Sau3AI and was ligated to cloning vector, LambdaGEM-ll. After in vitro packaging, DNA was used to infect E. coli KW 251. The genomic library was finally established in the plaque population. Recombinant phage DNAs containing the SOD gene were detected by a 32P-labelled synthetic oligonucleotide with 17 bases. The sequence of this oligonucleotide was deduced from the N-terminal amino acid sequence of B. fragilis FeSOD. Two recombinant phage DNAs were selected based on he results of plaque hybridization. Further analysis ith restriction mapping and DNA sequencing revealed that only one recombinant phage DNA contained the SOD gene.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access  LD5655.V856_1992.L355.pdf 6.51 Mb 00:30:08 00:15:30 00:13:34 00:06:47 00:00:34 next to an author's name indicates that all files or directories associated with their ETD are accessible from the Virginia Tech campus network only.

If you have questions or technical problems, please Contact DLA.