Title page for ETD etd-05072004-161023


Type of Document Master's Thesis
Author McKinney, Julie Michelle
Author's Email Address jusmith9@vt.edu
URN etd-05072004-161023
Title Identifying Sources of Fecal Pollution in the Appomattox River Watershed
Degree Master of Science
Department Crop and Soil Environmental Sciences
Advisory Committee
Advisor Name Title
Hagedorn, Charles III Committee Chair
Kern, James Committee Member
Knowlton, Katharine F. Committee Member
Keywords
  • TMDL
  • fecal bacteria
  • BST
  • Bacterial Source Tracking
Date of Defense 2004-04-28
Availability unrestricted
Abstract
Sources of E. coli were determined from impaired waterways in the Appomattox River watershed (in the lower Piedmont and South-Central Virginia) for the development of bacterial Total Maximum Daily Loads (TMDLs). The Appomattox River watershed is primarily undeveloped with 70.8% of the land forested, 17.0% used for agriculture (mainly livestock production), and 7.7% classified as water, wetland or barren land. The remaining 4.5% is developed for residential, commercial, and industrial land uses (mainly within the city of Petersburg).

Using Antibiotic Resistance Analysis, a known source library of 1,280 E. coli isolates (320 isolates per source) was constructed. Water samples were collected monthly for between eleven and fourteen months (11/02-12/03) from 40 locations throughout the Appomattox watershed and analyzed for fecal coliforms, E. coli, and resistance to 7 antibiotics of varying concentrations. A total of 486 water samples (9,907 isolates) were analyzed during the study. The objectives of this study were verify that each sampling site exceeded state bacterial count standards (using fecal coliform data), to compare the Discriminate Analysis and Logistic Regression statistical models for use in the classification of isolates, and finally to determine the source of contamination at each site.

The fecal coliform and E.coli data was used to determine if each site exceeded state standards during the assessment period. Thirty-eight of the sites exceeded the fecal coliform standard at least 10% of the time, and thirty-three exceeded the E.coli standard at least 10% of the time.

Discriminate Analysis (DA) is typically used to classify isolates, but the results obtained from the DA model were unrealistic based on the watershed land uses. By statistically analyzing the original 1,280 E.coli isolates six different ways, a more appropriate classification of isolates was determined. The six analyzing methods were Regular DA and Logistic Regression (LR); DA and LR where each isolate whose probability fell below 80% was deleted; DA and LR where each isolate whose probability fell below 80% was used to create an Unknown category. The Logistic Regression model with an Unknown category proved to be the most appropriate. By using the Logistic Regression model, with Unknown category, to classify isolates, twenty five of the forty sites were discovered to be contaminated predominately with Livestock and fourteen of the sites predominately by Wildlife. One site was equally divided between these two categories. Human and Pet contamination were not dominant at any of the forty sites.

This comparison of the DA and LR statistical methods could change the analysis standard for Bacterial Source Tracking and suggests that the model required to classify isolates depends on the watershed characteristics.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Abstract.pdf 67.11 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01
  Acknowledgements.pdf 48.30 Kb 00:00:13 00:00:06 00:00:06 00:00:03 < 00:00:01
  AppendixA.pdf 435.37 Kb 00:02:00 00:01:02 00:00:54 00:00:27 00:00:02
  AppendixB.pdf 2.04 Mb 00:09:26 00:04:51 00:04:14 00:02:07 00:00:10
  AppendixC.pdf 655.13 Kb 00:03:01 00:01:33 00:01:21 00:00:40 00:00:03
  ChapterI.pdf 106.30 Kb 00:00:29 00:00:15 00:00:13 00:00:06 < 00:00:01
  ChapterII.pdf 124.74 Kb 00:00:34 00:00:17 00:00:15 00:00:07 < 00:00:01
  ChapterIII.pdf 390.93 Kb 00:01:48 00:00:55 00:00:48 00:00:24 00:00:02
  ChapterIV.pdf 433.43 Kb 00:02:00 00:01:01 00:00:54 00:00:27 00:00:02
  ChapterV.pdf 73.96 Kb 00:00:20 00:00:10 00:00:09 00:00:04 < 00:00:01
  ListofFigures.pdf 96.98 Kb 00:00:26 00:00:13 00:00:12 00:00:06 < 00:00:01
  ListofTables.pdf 151.77 Kb 00:00:42 00:00:21 00:00:18 00:00:09 < 00:00:01
  TableofContents.pdf 72.25 Kb 00:00:20 00:00:10 00:00:09 00:00:04 < 00:00:01
  TitlePage.pdf 45.86 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01
  Vitae.pdf 44.95 Kb 00:00:12 00:00:06 00:00:05 00:00:02 < 00:00:01

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