Title page for ETD etd-05132010-235423


Type of Document Master's Thesis
Author Sheets, Kevin
Author's Email Address sheetsk@vt.edu
URN etd-05132010-235423
Title Mechanical and Cellular Response to Biomineralization of Ovalbumin Scaffolds for Bone Tissue Engineering
Degree Master of Science
Department Materials Science and Engineering
Advisory Committee
Advisor Name Title
Morgan, Abby W. Committee Chair
Corcoran, Sean Gerald Committee Member
Riffle, Judy S. Committee Member
Keywords
  • simulated body fluid
  • tissue engineering
  • calcium phosphate
  • ovalbumin
Date of Defense 2010-05-03
Availability unrestricted
Abstract
Studies regarding the feasibility of ovalbumin (OVA) as a bone scaffold material have found its cost, availability, interaction with cells, and ability to degrade in the body into safe byproducts to be ideal for such an application. However, weak mechanical properties cause hesitation in the use of OVA as a scaffolding material in much stronger native tissue. To enhance the mechanical strength of the OVA scaffolds without compromising in vitro cellular performance, Ca-P crystals were grown on unmodified OVA and phosphonated OVA (p-OVA) samples via biomineralization processes using 5x-concentrated simulated body fluid (5x SBF).

Electron microscopy (ESEM/EDS) data confirm the formation of Ca-P crystals on the surface of OVA and p-OVA scaffolds. Mechanically, rheology data measured a minimum of a three-fold increase in each mineralized scaffold’s complex shear modulus over unmineralized counterparts. Degradation in a PBS+collagenase XI environment showed that mineralization extended total time to degradation. It was also shown that the formation of the Ca-P crystals had no negative effects on in vitro cell studies. To measure cellular response, a live/dead assay was conducted to confirm cell viability after 24 hours.

In conclusion, improvements were made to mechanical strength without compromising in vitro cell-scaffold response. While it remains unknown whether the increase in strength is adequate for use as a bone scaffold, future work should focus on gathering necessary information to study OVA scaffolds in animal models for eventual consideration as a bone graft substitute material.

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