Title page for ETD etd-05242002-165327


Type of Document Master's Thesis
Author Varrieur, John Michael
Author's Email Address jvarrieu@vt.edu
URN etd-05242002-165327
Title AFLP Marker Analysis Of Monoploid Potato
Degree Master of Science
Department Horticulture
Advisory Committee
Advisor Name Title
Veilleux, Richard E. Committee Chair
Buss, Glenn R. Committee Member
Jelesko, John G. Committee Member
Keywords
  • AFLP
  • monoploid
  • haploid
  • potato
  • Solanum phureja
Date of Defense 2002-05-17
Availability unrestricted
Abstract
ABSTRACT

Potato haploids have been recent components in protoplast fusion research, strategies to combine wild and cultivated potato germplasm and the generation of economically valuable mutant phenotypes. Additionally, most major genetic mapping and QTL analyses in potato have utilized haploid germplasm to simplify linkage-mapping computations. The accuracy of genetic assumptions concerning the randomness and genetic purity of haploid genomes may directly affect the statistical validity of many results in current potato research. In the present study, AFLP analysis was conducted on two sibling S. phureja "BARD 1-3" monoploid populations derived by androgenesis in anther culture, and gynogenesis through the use of a haploid-inducing pollinator, S. phureja "IVP 101." Little indication of somaclonal variation and haploid-inducer gene introgression was found in the monoploid band data suggesting genomic stability. Segregation of marker alleles that were heterozygous in the parent was distorted from the expected 1:1 ratio in both populations, ranging from 35% in the gynogenic monoploids (GM) to 46% in the androgenic monoploids (AM). Genetic diversity appeared more random among the monoploid populations after skewed marker data was removed from phylogenetic analyses. Bilateral and unilateral marker skewness in the monoploid populations may respectively indicate common and unique segregation distorting loci (SDL) present in the AM and GM genomes. Representatives of both SDL types were located on a partial linkage map created using androgenic monoploid data.

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