Type of Document	Master's Thesis
Author	Gordon, Gale Ross
URN	etd-06102012-040053
Title	The isolation and characterization of a growth factor in rumen fluid for a strain of Butyrivibrio
Degree	Master of Science
Department	Biochemistry
Advisory Committee	Advisor Name Title Moore, W. E. C. Committee Chair Fulghum, R. E. Committee Member King, Kendall W. Committee Member Kramer, Clyde Y. Committee Member
Keywords	Butyrivibrio
Date of Defense	1961-05-05
Availability	restricted
Abstract One or more factors which occur in bovine rumen fluid stimulate the growth of a strain of Butyrivibrio. The stimulating material is heat stable, organic in nature and non-dialyzable. It cannot be extracted from rumen fluid with lipid solvents and is retained in part on anion and cation exchange resins. It can be eluted from the resins with strong acid. It is stable to enzymatic hydrolysis by trypsin. Granular mucin or bovine saliva will partially replace the stimulatory activity. The part of the material which was not replaced by mucin did not appear to be any compound that is commonly used to stimulate bacterial growth. The presence of a possible inhibitor for the growth of a strain of Butyrivibrio was demonstrated.
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