Type of Document Master's Thesis Author Shannon, Robert David URN etd-06102012-040255 Title Evaluation of a mitochondrial test for the determination of chemical toxicity Degree Master of Science Department Environmental Sciences and Engineering Advisory Committee
Advisor Name Title Boardman, Gregory D. Committee Chair Bevan, David R. Committee Member Dietrich, Andrea M. Committee Member Hoehn, Robert C. Committee Member Keywords
- Toxicity testing.
Date of Defense 1988-07-15 Availability restricted AbstractThe feasibility of using rat liver mitochondria respiratory parameters
as a short-term toxicity test was investigated. Mitochondrial fractions were
exposed to six concentrations of five chlorophenols. Respiratory parameters
were measured and compared to control experiments. The toxicity of the
chlorophenos, measured by the 50% uncoupling concentration (UC50),
increased with increasing chloro substitution.
The UC50 values for the five chlorophenols were compared to six
physicochemical parameters for the same chlorophenols and high degrees of
correlation were found (r >/- 0.890). The highest correlation coefficient
obtained was with the octanol-water partition coefficient. UC50 values were
also compared to nine currently existing short-term toxicity tests. High
degrees of correlation were obtained with several of these tests, including
bacterial and fish bioassays.
From the results of these experiments, the measurement and use of
mitochondria respiratory parameters as a short-term toxicity test appears to
offer an alternative to currently used short-term toxicity tests, particularly
with chemicals having physicochemical characteristics similar to
Filename Size Approximate Download Time (Hours:Minutes:Seconds)
28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access LD5655.V855_1988.S423.pdf 7.30 Mb 00:33:48 00:17:23 00:15:12 00:07:36 00:00:38next to an author's name indicates that all files or directories associated with their ETD are accessible from the Virginia Tech campus network only.
If you have questions or technical problems, please Contact DLA.