Type of Document	Master's Thesis
Author	Shannon, Robert David
URN	etd-06102012-040255
Title	Evaluation of a mitochondrial test for the determination of chemical toxicity
Degree	Master of Science
Department	Environmental Sciences and Engineering
Advisory Committee	Advisor Name Title Boardman, Gregory D. Committee Chair Bevan, David R. Committee Member Dietrich, Andrea M. Committee Member Hoehn, Robert C. Committee Member
Keywords	Toxicity testing.
Date of Defense	1988-07-15
Availability	restricted
Abstract The feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. Mitochondrial fractions were exposed to six concentrations of five chlorophenols. Respiratory parameters were measured and compared to control experiments. The toxicity of the chlorophenos, measured by the 50% uncoupling concentration (UC50), increased with increasing chloro substitution. The UC50 values for the five chlorophenols were compared to six physicochemical parameters for the same chlorophenols and high degrees of correlation were found (r >/- 0.890). The highest correlation coefficient obtained was with the octanol-water partition coefficient. UC50 values were also compared to nine currently existing short-term toxicity tests. High degrees of correlation were obtained with several of these tests, including bacterial and fish bioassays. From the results of these experiments, the measurement and use of mitochondria respiratory parameters as a short-term toxicity test appears to offer an alternative to currently used short-term toxicity tests, particularly with chemicals having physicochemical characteristics similar to mitochondria uncouplers.
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