Type of Document	Master's Thesis
Author	Hewes, Christina Andrea
URN	etd-06142006-193636
Title	Characterization of Apoptotic Cells in Equine Proximal Suspensory Desmitis
Degree	Master of Science
Department	Biomedical and Veterinary Sciences
Advisory Committee	Advisor Name Title White, Nathaniel A. II Committee Chair Buechner-Maxwell, Virginia A. Committee Member Robertson, John L. Committee Member
Keywords	desmitis horse apoptosis suspensory ligament
Date of Defense	2006-05-30
Availability	restricted
Abstract Suspensory desmitis is a common problem and affects a broad cross section of equine athletes in various disciplines. For this study, the proximal portion of the suspensory ligament was collected from 6 horses without suspensory ligament injury (16 ligaments) and 4 horses with degeneration of the suspensory ligament (11 ligaments). Specimens were collected immediately after euthanasia and placed in neutral-buffered 10% formalin. The tissue was fixed, sectioned, and stained with hematoxylin and eosin (H&E), Masson’s trichrome, and for apoptosis by the terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique. Histological changes in the abnormal ligaments included mineralization, fibroplasias, neovascularization, collagen degeneration, and significant architecture disruption in 2 ligaments. There was a trend for increased apoptosis in the injured ligaments compared to the normal ligaments.
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