| Type of Document |
Master's Thesis |
| Author |
Leininger, Dagny Jayne
|
| Author's Email Address |
dairycowdoc@hotmail.com |
| URN |
etd-06152001-174539 |
| Title |
Escherichia coli Mastitis in the Dairy Bovine |
| Degree |
Master of Science |
| Department |
Veterinary Medical Sciences |
| Advisory Committee |
| Advisor Name |
Title |
| Roberson, Jerry R. |
Committee Chair |
| Akers, Robert Michael |
Committee Member |
| Elvinger, Francois C. |
Committee Member |
| Hovingh, Ernest P. |
Committee Member |
|
| Keywords |
- MAstitis
- Escherichia coli
- Esoin Methylene BLue Agar
- Frequent Milk-out
|
| Date of Defense |
2001-05-09 |
| Availability |
unrestricted |
Abstract
Diagnosis techniques and treatments for Escherichia coli mastitis in the dairy bovine were evaluated in two experiments. The first experiment evaluated eosin methylene blue agar as a method of distinguishing E.coli from other gram-negative mastitis pathogens. Escherichia coli will usually produce a green metallic sheen on eosin methylene blue agar. One hundred and twenty-nine milk samples or gram-negative isolates from milk samples were used to compare eosin methylene blue agar to a commercial biochemical test strip (the accepted standard). There was an intermethod agreement of 96.9% and a k-value of 93.7% indicating excellent agreement beyond chance between test methods. Eosin methylene blue agar is a reliable method for differentiation of E. coli from other gram-negative mastitis pathogens. The second experiment evaluated the efficacy of frequent milk-out as a treatment for E. coli mastitis. Sixteen Holstein dairy cows were divided into 2 blocks and randomly assigned to 1 of 4 treatment groups: 1) non-infected, not frequently milked-out, i.e. not treated (NI-NT), 2) experimentally infected with E. coli, not treated (EC-NT), 3) non-infected, frequently milked-out (NI-FMO), and 4) experimentally infected with E. coli, frequently milked-out (EC-FMO). Hours to bacterial, clinical and systemic cure were not different between the EC-NT and EC-FMO treatment groups. Serum a-lactalbumin concentrations were evaluated between treatment groups as a measure of udder health. Serum a-lactalbumin concentrations were higher in cows in the EC-NT treatment group than cows in the NI-NT, NI-FMO and EC-FMO treatment groups at 12 hours post-experimental challenge. Serum a-lactalbumin concentrations were higher in cows in the NI-FMO treatment group than in cows in the NI-NT, EC-NT and EC-FMO treatment groups at 36 hours post-experimental challenge. Results from this study do not support frequent milk-out as a treatment for E. coli mastitis.
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LeiningerThesis.pdf |
31.07 Mb |
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