Type of Document Master's Thesis Author Sumners, Lindsay Hart Author's Email Address firstname.lastname@example.org URN etd-06202011-123953 Title Immunological Response to Clostridium perfringens in Two Genetically Divergent Lines of Chickens as Influenced by Major Histocompatibility Complex (MHC) Genotype Degree Master of Science Department Animal and Poultry Sciences Advisory Committee
Advisor Name Title Dalloul, Rami A. Committee Chair McElroy, Audrey P. Committee Member Wong, Eric A. Committee Member Keywords
- immune response
- genetic resistance
- necrotic enteritis
- Clostridium perfringens
Date of Defense 2011-06-06 Availability restricted AbstractChickens genetically selected for low (LA) or high (HA) antibody response to sheep red blood
cells (SRBC) displayed a correlated change in major histocompatibility complex (MHC), so that
LA chickens were 96% B13 and HA chickens were 96% B21. During a clinical outbreak of
necrotic enteritis, B21B21 genotypes experienced significantly less mortality (6% vs. 13 %)
compared to B13B13 genotypes. A study was carried out to assess immunological differences
between LA and HA lines during exposure to Clostridium perfringens. In Experiment 1,
chickens were orally gavaged with a low (10^7 CFU/mL) or high (10^9 CFU/mL) dose of C.
perfringens. In Experiment 2, chickens were orally gavaged with live coccidia oocysts on
experiment d 1, followed by 107 CFU/mL C. perfringens on d 5. Unfortunately, establishment of
necrotic enteritis infection was unsuccessful in both experiments as evidenced by lack of
significant intestinal lesions, as well as no negative effect on bird performance. In an ex vivo
study, peripheral blood mononuclear cells (PBMCs) were isolated from each genetic line,
cultured, stimulated with LPS (4 h), and exposed to varying concentrations of C. perfringens α-
toxin (1, 10, 100, 1000 U/L) for 2 and 4 h. Evaluation of cellular proliferation, percent
cytotoxicity and immunological gene expression was carried out in a variety of experiments.
Genetic lines were found to be highly divergent in all analyses.
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