Title page for ETD etd-07122000-16230006

Type of Document

Master's Thesis

Author

Pung, Thitiya

Author's Email Address

tpung@vt.edu

URN

etd-07122000-16230006

Title

The Isolation of Natural Products From Plant Extracts

Degree

Master of Science

Department

Chemistry

Advisory Committee

Advisor Name	Title
Kingston, David G. I.	Committee Chair
Gandour, Richard D.	Committee Member
Hanson, Brian E.	Committee Member
Tanko, James M.	Committee Member

Keywords

oxoaporphine
verazines
natural product
biochanin A
DNA-damaging agents

Date of Defense

2000-06-28

Availability

unrestricted

Abstract

Bioassay-guided fractionation of the ethyl acetate extract of Virola sp.
(Myristicaceae) using the Sc7 yeast strain resulted in the isolation of the weakly cytotoxic
biochanin A (an isoflavone compound). The bioassay of three mutant yeast strains
(1138, 1104, and 1353) directed the isolation of DNA-damaging alkaloids from Solanum
hostmannii. These alkaloids were identified as verazine and (20R) epimer verazine. In
addition, three oxoaporphine alkaloids were isolated from the bark of Papualthia sp.
(Annonaceae). Oxocrebanine and atherospermidine showed DNA-damaging activity
whereas liriodenine had cytotoxic activity.

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