Title page for ETD etd-07142011-082442


Type of Document Master's Thesis
Author Landa, Chelsea E
Author's Email Address clanda@vt.edu
URN etd-07142011-082442
Title Evaluation of Weaning Stress in Beef Calves
Degree Master of Science
Department Biomedical and Veterinary Sciences
Advisory Committee
Advisor Name Title
Swecker, William S. Jr. Committee Chair
Tracy, Benjamin Franklin Committee Member
Wahlberg, Mark L. Committee Member
Keywords
  • IgG2
  • IgG1
  • IFNγ
  • blood glucose
  • qPCR
  • forage-finished beef
  • calves
  • weaning
  • fenceline
  • cattle
Date of Defense 2011-06-30
Availability unrestricted
Abstract
Conventional techniques within the beef cattle industry involve weaning the calf from the dam when the calf is about 205 days of age. Weaning induces a stress-response that is implicated in reducing the health and productivity of newly weaned calves. Our goal was to evaluate the impact of weaning on the stress immune responses of beef calves. To that end, we 1) evaluated novel methods to quantify physiological markers of stress, 2) compared immune function and growth of calves grazing legume versus grass forages, and 3) compared the effects of abrupt versus two-stage weaning on calves. In study 1, calf, yearling, and adult beef cattle were used to assess the accuracy and precision of handheld glucometers in quantifying bovine blood glucose concentration. Precision Xtra® and ReliOn® glucometers were used chute side to quantify blood glucose concentrations in cattle and were compared to an accepted plasma glucose analysis on the same samples for validation. The Precision Xtra® glucometer was more accurate and precise than the ReliOn® glucometer. In study 2, weaned heifers were used to compare the immunomodulatory effects of grazing alfalfa versus fescue over a 30 day grazing period. No differences were detected in the interferon gamma (IFNγ) production and weight gain between the heifers on alfalfa and fescue. In study 3, effects of two-stage (fenceline) and abrupt weaning were compared. Calf weights, immune cell function, antibody production, blood glucose concentrations, fecal cortisol concentrations, and gene expression (FAS, IL-4,IL-10, and IFNγ) were measured pre- and post-weaning. On the day after weaning, the abruptly weaned calves had higher blood glucose concentrations than fenceline weaned calves. Fecal cortisol

concentration and gene expression of FAS and IL-4 increased in both groups after weaning, but no differences were detected between the weaning treatments. Gene expression of IL-10 and IFNγ did not change over time. No date, treatment or treatment*date effect was detected for total weight gain or IFNγ production within the non-stimulated and the mitogen-stimulated whole blood samples.

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