Type of Document	Master's Thesis
Author	Johnson, Larry
URN	etd-07152010-020103
Title	Factors affecting preservation of liquid and frozen ram spermatozoa.
Degree	Master of Science
Department	Animal Science
Advisory Committee	Advisor Name Title Meacham, Thomas N. Committee Chair Saacke, Richard G. Committee Member Wise, Milton B. Committee Member
Keywords	Frozen semen.
Date of Defense	1974-07-15
Availability	restricted
Abstract A comparison of the results of Experiments 1 and 2 vividly demonstrates the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress, there was more variation among treatments reflected in maintenance of both intact acrosomes and motile life (Experiment 2, Table 9) than when unfrozen sperm were studied (Experiment 1, Table 5). The influence of glycerol and Tris are particularly noteworthy. Though rate of thaw is not part of the surrounding media, it does control the amount of time the cells are subjected to an even more hostile environment (high salt concentrations) encountered near the melting point of ice. Therefore, the benefit of higher thaw temperatures and resulting faster thaw rates was undoubtedly due to minimizing exposure time of spermatozoa to this adverse condition.
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