Title page for ETD etd-07152010-020103
|Type of Document
||Factors affecting preservation of liquid and frozen ram spermatozoa.
||Master of Science
|Meacham, Thomas N.
|Saacke, Richard G.
|Wise, Milton B.
|Date of Defense
A comparison of the results of Experiments 1 and 2 vividly demonstrates
the vulnerability of ram spermatozoa to the stress of freeze-thawing. When ram spermatozoa were subjected to freeze-thaw stress,
there was more variation among treatments reflected in maintenance of
both intact acrosomes and motile life (Experiment 2, Table 9) than when
unfrozen sperm were studied (Experiment 1, Table 5). The influence of
glycerol and Tris are particularly noteworthy. Though rate of thaw is
not part of the surrounding media, it does control the amount of time
the cells are subjected to an even more hostile environment (high salt
concentrations) encountered near the melting point of ice. Therefore,
the benefit of higher thaw temperatures and resulting faster thaw rates
was undoubtedly due to minimizing exposure time of spermatozoa to this adverse condition.
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