Title page for ETD etd-07212010-020434


Type of Document Master's Thesis
Author Frey, Randall Scott
URN etd-07212010-020434
Title Characterization of estrogen and glucocorticoid receptors, skeletal muscle protein turnover and tissue growth in lambs treated with trenbolone acetate and estradiol
Degree Master of Science
Department Animal Science
Advisory Committee
Advisor Name Title
McCarthy, F. D. Committee Chair
Akers, Robert Michael Committee Member
Eversole, Dan E. Committee Member
Webb, Kenneth E. Jr. Committee Member
Keywords
  • Estrogen
Date of Defense 1988-02-15
Availability restricted
Abstract

A study was conducted to determine the effects of trenbolone acetate (TBA) and estradiol-17B (E2) implantation on the characteristics of the glucocorticoid and E2 receptor, skeletal muscle protein turnover and tissue growth. Twenty-four lambs were utilized. Trenbolone acetate did not ,affect (P>.10) degradation rates in the semitendinosus (ST) and triceps brachii (TB) muscles, the production of cortisol, adrenal weights and cytosolic glucocorticoid binding capcity (Bmax). Trenbolone acetate decreased synthesis rate of muscle protein (P<.Ol), the percent of [3H] dexamethasone binding in the nuclear fraction, Bmax and the disociation constant (Kd) of the cytosolic E2 receptor, only in the TB muscle.

Deoxyribonucleic acid (DNA) of the TB was increased (P<.05) with TBA. Pituitary weights were decreased (P<.005) with TBA and increased (P<.Ol) with E2. Estradiol decreased (P<.05) Bmax of the cytosolic E2 receptor in the ST and decreased (P<.05) Bmax of the nuclear E2 receptor in the TB muscle. The TB muscle had greater (P<.05) synthesis rates than the ST and the protein:RNA ratio was decreased (P<.05) in the TB. The TB muscle had greater (P<.005) Bmax for the cytosolic glucocorticoid receptor.

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