| Type of Document |
Master's Thesis |
| Author |
Schuchert, Jennifer Ann
|
| URN |
etd-07242002-214305 |
| Title |
Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction |
| Degree |
Master of Science |
| Department |
Veterinary Medical Sciences |
| Advisory Committee |
| Advisor Name |
Title |
| Inzana, Thomas J. |
Committee Chair |
| Boyle, Stephen M. |
Committee Member |
| Sriranganathan, Nammalwar |
Committee Member |
|
| Keywords |
- Multiplex PCR
- serotyping
- Actinobacillus pleuropneumoniae
|
| Date of Defense |
2002-06-26 |
| Availability |
unrestricted |
Abstract
Traditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8.
|
| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
|
| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
| |
finalThesis082602.pdf |
856.11 Kb |
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00:02:02 |
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