Type of Document Master's Thesis Author Murphy, Stephanie A. URN etd-07242012-040202 Title Effects of selenium and vitamin B-6 on growth of chemically- induced transplanted tumors in BALB/c inbred mice Degree Master of Science Department Human Nutrition and Foods Advisory Committee
Advisor Name Title Driskell, Judy A. Committee Chair Blodgett, Dennis J. Committee Member Elgert, Klaus D. Committee Member Ritchey, Sanford J. Committee Member Keywords
Date of Defense 1989-03-30 Availability restricted AbstractMale weanling inbred, mice were inoculated with
fibrosarcoma cells (hindquarter) originally produced by 2-methylcholanthrene. Before inoculation, mice were randomly divided into three groups of 24 and one of 12
(control). After a one week acclimation period, each group was fed a diet containing either suboptimal vitamin B-6, 0.5 mg/kg diet; adequate, 7.0 mg/kg diet; or excess, 100 mg/kg diet. Controls were fed the adequate vitamin. B-6 diet. Twenty-four hours after
tumor cell inoculation, a series of sodium selenite injections (0.5 μg/.10 mL) were given to half of each treatment group and all controls. Mice were sacrificed
two wk after tumor inoculation. Tumors were excised and weighed. Selenium-treated mice had significantly smaller tumors as compared to untreated mice regardless
of vitamin B-6 treatment. The smallest tumors were
found in the selenium—treated group maintained on
adequate B-6, while the largest tumors were developed
by mice on the excess B-6 diet without selenium treatments.
All groups had similar blood selenium levels as
measured by gas chromatography. Tumor selenium levels,
analyzed by atomic absorption, were significantly
higher for untreated groups than selenium—treated
groups (larger tumor size). The excess and adequate
vitamin B-6 selenium-treated groups had significantly
lower tumor selenium levels than the adequate vitamin
B-6 untreated group. Plasma pyridoxal phosphate
(concentrations) determined radiometrically and tumor
vitamin B-6 levels determined microbiologically, related directly to dietary treatments. Sodium selenite
injections and adequate vitamin B-6 diets reduced the
size of fibrosarcomas in BALB/c inbred mice.
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