Title page for ETD etd-07282010-020224
|Type of Document
||Guilfoyle, John Rohan
||Effect of transport methods on recovery of bacteria from ground beef samples.
||Master of Science
||Food Science and Technology
|Pierson, Merle D.
|Kotula, A. W.
|Lechowich, R. V.
|Date of Defense
A comparison of 2 basic methods presently used to transport
perishable food samples intended for microbiological analysis is
presented. A transport system using an appropriate, pre-chilled container
with dry ice as the temperature contro11ant resulted in a significant1y
higher (P ~ 0.01) survival rate of Clostridium perfringens
vegetative cells in ground beef samples as compared to survivals in
samples shipped in Trans Temp containers using canisters of a commercia11y
formulated eutectic salts mixture as temperature contro1lant.
Dry ice kept samples also resulted in greater recoveries of
Staphylococcus aureus and the aerobic microf1ora~ Slightly higher
recoveries of co1iforms and Escherichia coli were obtained from samples held by the Trans Temp procedure! Mixing samples 1:1 with 10, 20 or
30% (wt/vo1) buffered solutions of either dimethylsulfoxide or glycerol
prior to freezing generally improved the survival of all microorganisms
assayed regardless of the transport system tested. The results indicate
that packaging perishable food samples with a volume of cryoprotective
solute may be a useful adjunct to frozen transport systems thereby
improving survival and allowing more complete recovery of selective
micro-rganisms with existing assay procedures.
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