Title page for ETD etd-07282010-020246


Type of Document Master's Thesis
Author Haynes, Linda Rose
URN etd-07282010-020246
Title Alkylation of rat lens crystallins with iodoacetamide
Degree Master of Science
Department Biochemistry and Nutrition
Advisory Committee
Advisor Name Title
Hess, John L. Committee Chair
Barnett, Lewis B. Committee Member
Bunce, George Edwin Committee Member
Rutherford, Charles L. Committee Member
Keywords
  • Alkylation
Date of Defense 1977-09-05
Availability restricted
Abstract
Alkylation of lens proteins with iodoacetamide during homogenization of tissue (50 millimolar excess) immediately followed by gel-permeation chromatography yielded a crystallin population devoid of βH-crystallin. This result occurred in lens homogenates from both young (100 g) and older (400 g) male rats. BetaH-crystallin was not converted to insoluble protein with alkylation. Each crystallin fraction reacted with radioactive iodoacetamide in proportion to sulfhydryl content; at a ratio of 1 mg iodoacetamide/mg protein total free-sulfhydryl of the crystallins had reacted after 1 hr at pH 8, 25°C. Alkylated α-, βL-' and y-crystallin fractions demonstrated no altered chromatographic behavior on Sephacryl S-200; only alkylated βH-crystallin was altered so that it co-chromatographed with control or alkylated βL-crystallin.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[VT] LD5655.V855_1977.H39.pdf 11.66 Mb 00:53:57 00:27:45 00:24:16 00:12:08 00:01:02
[BTD] next to an author's name indicates that all files or directories associated with their ETD are accessible from the Virginia Tech campus network only.

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.