Title page for ETD etd-080999-101905


Type of Document Master's Thesis
Author Hensley, Erika L.
Author's Email Address ehensley@vt.edu
URN etd-080999-101905
Title Induced Cervical Dilation in Sheep: Evaluation of the Effects On fertilization Rates and Embryonic Development
Degree Master of Science
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
Lewis, Gregory S. Committee Chair
Hall, John B. Committee Member
Saacke, Richard G. Committee Member
Keywords
  • Cervix
  • Oxytocin
  • Sheep
Date of Defense 1999-07-12
Availability restricted
Abstract
Experiments were conducted to determine the effects of a treatment used to dilate the

cervix for transcervical AI and embryo transfer on pregnancy or fertilization rates in sheep.

Experiment 1 was conducted as a 2 x 2 factorial, with the main effects of estradiol-17b (E2) and

oxytocin (OT). On d 6 of pregnancy, ewes received (i.v.) 100 mg of estradiol-17b, or saline; 10 h

later, ewes received (i.v.) 400 USP units (20 mL) or saline. Blood samples were collected for

progesterone analysis. Transrectal ultrasonography was used to determine pregnancy on d 25.

Treatment did not affect (c 2 , P > .05) pregnancy rates. For nonpregnant ewes, the

period × E2 × OT interaction (P < .01) was significant.

Experiments were conducted to evaluate the effects of oxytocin and(or) cervical manipulation on fertilization rates. In Exp. 2, nulliparous ewes were

assigned to one of two randomized treatment groups: 1) saline or 2) oxytocin. In Exp. 3, multiparous ewes were assigned to one of two randomized treatment groups: 1) saline-cervical

manipulation or 2) oxytocin-cervical manipulation. Ewes in Exp. 2 and 3 were laparoscopically

inseminated. In Exp. 2 and 3, ewes received (i.v.) either 400 USP units of oxytocin or 20 mL of

saline. In Exp. 2 and 3, oocytes and(or) embryos were collected. Treatment did not affect

recovery, fertilization, or pregnancy rates in Exp.2 and 3. In conclusion, the treatment used to

dilate the cervix in ewes to facilitate the transcervical AI or ET does not seem to affect

fertilization or pregnancy rates.

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