Title page for ETD etd-09062007-000547


Type of Document Master's Thesis
Author Karkare, Sampada S
URN etd-09062007-000547
Title Isolation and Structure Elucidation of Antiproliferative Agents From Madagascar Rainforests
Degree Master of Science
Department Chemistry
Advisory Committee
Advisor Name Title
Kingston, David G. I. Committee Chair
Brewer, Karen J. Committee Member
Carlier, Paul R. Committee Member
Keywords
  • Strophanthus
  • triterpenoids
  • Grewia sp.
  • Roupellina
  • Grewia
  • boivinide
  • cardenolide glycosides
  • A2780
  • natural products
Date of Defense 2007-08-30
Availability unrestricted
Abstract
Through our continuing search for anticancer agents from Madagascar rainforests as a part of International Cooperative Biodiversity Group (ICBG), we received two extracts which were active against the A2780 human ovarian cancer cell line and hence were selected for further fractionation. Six compounds were isolated from these extracts. The structure elucidation and characterization of these compounds was carried out using mass spectrometry and 1D and 2D NMR techniques.

The bioassay-guided fractionation of Roupellina (Strophanthus) boivinii yielded three new and one known cardenolide glycosides. The structure of the known cardenolide glycoside was determined after comparison of NMR data to that found in literature for digitoxigenin 3-O-β-D-glucopyranosyl-(1→4)-α-L-acofriopyranoside. All four compounds exhibited good antiproliferative activity on the A2780 bioassay.

The fractionation of the extract of Grewia sp. led to the isolation of one new and one known triterpenoid. The known triterpenoid was identified as 7β-hydroxy-23-deoxojessic acid and its structure was confirmed by comparison of its 1D and 2D NMR data to that found in literature.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Abbreviations.pdf 6.83 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  Acknowledgements.pdf 7.33 Kb 00:00:02 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  Contents.pdf 63.48 Kb 00:00:17 00:00:09 00:00:07 00:00:03 < 00:00:01
  Figures.pdf 65.36 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01
  Schemes.pdf 6.95 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  Supporting_information_chap_2.pdf 747.80 Kb 00:03:27 00:01:46 00:01:33 00:00:46 00:00:03
  Supporting_information_chap_3.pdf 637.33 Kb 00:02:57 00:01:31 00:01:19 00:00:39 00:00:03
  Tables.pdf 7.88 Kb 00:00:02 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  Thesis-chap_1.pdf 253.70 Kb 00:01:10 00:00:36 00:00:31 00:00:15 00:00:01
  Thesis-chap_2.pdf 429.65 Kb 00:01:59 00:01:01 00:00:53 00:00:26 00:00:02
  Thesis-chap_3.pdf 252.76 Kb 00:01:10 00:00:36 00:00:31 00:00:15 00:00:01
  Thesis-chap_4.pdf 70.49 Kb 00:00:19 00:00:10 00:00:08 00:00:04 < 00:00:01
  Thesis-chap_5.pdf 88.75 Kb 00:00:24 00:00:12 00:00:11 00:00:05 < 00:00:01
  Title_abstract.pdf 93.90 Kb 00:00:26 00:00:13 00:00:11 00:00:05 < 00:00:01

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.