Type of Document	Master's Thesis
Author	Karkare, Sampada S
URN	etd-09062007-000547
Title	Isolation and Structure Elucidation of Antiproliferative Agents From Madagascar Rainforests
Degree	Master of Science
Department	Chemistry
Advisory Committee	Advisor Name Title Kingston, David G. I. Committee Chair Brewer, Karen J. Committee Member Carlier, Paul R. Committee Member
Keywords	Strophanthus triterpenoids Grewia sp. Roupellina Grewia boivinide cardenolide glycosides A2780 natural products
Date of Defense	2007-08-30
Availability	unrestricted
Abstract Through our continuing search for anticancer agents from Madagascar rainforests as a part of International Cooperative Biodiversity Group (ICBG), we received two extracts which were active against the A2780 human ovarian cancer cell line and hence were selected for further fractionation. Six compounds were isolated from these extracts. The structure elucidation and characterization of these compounds was carried out using mass spectrometry and 1D and 2D NMR techniques. The bioassay-guided fractionation of Roupellina (Strophanthus) boivinii yielded three new and one known cardenolide glycosides. The structure of the known cardenolide glycoside was determined after comparison of NMR data to that found in literature for digitoxigenin 3-O-β-D-glucopyranosyl-(1→4)-α-L-acofriopyranoside. All four compounds exhibited good antiproliferative activity on the A2780 bioassay. The fractionation of the extract of Grewia sp. led to the isolation of one new and one known triterpenoid. The known triterpenoid was identified as 7β-hydroxy-23-deoxojessic acid and its structure was confirmed by comparison of its 1D and 2D NMR data to that found in literature.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access    Abbreviations.pdf 6.83 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01   Acknowledgements.pdf 7.33 Kb 00:00:02 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01   Contents.pdf 63.48 Kb 00:00:17 00:00:09 00:00:07 00:00:03 < 00:00:01   Figures.pdf 65.36 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01   Schemes.pdf 6.95 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01   Supporting_information_chap_2.pdf 747.80 Kb 00:03:27 00:01:46 00:01:33 00:00:46 00:00:03   Supporting_information_chap_3.pdf 637.33 Kb 00:02:57 00:01:31 00:01:19 00:00:39 00:00:03   Tables.pdf 7.88 Kb 00:00:02 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01   Thesis-chap_1.pdf 253.70 Kb 00:01:10 00:00:36 00:00:31 00:00:15 00:00:01   Thesis-chap_2.pdf 429.65 Kb 00:01:59 00:01:01 00:00:53 00:00:26 00:00:02   Thesis-chap_3.pdf 252.76 Kb 00:01:10 00:00:36 00:00:31 00:00:15 00:00:01   Thesis-chap_4.pdf 70.49 Kb 00:00:19 00:00:10 00:00:08 00:00:04 < 00:00:01   Thesis-chap_5.pdf 88.75 Kb 00:00:24 00:00:12 00:00:11 00:00:05 < 00:00:01   Title_abstract.pdf 93.90 Kb 00:00:26 00:00:13 00:00:11 00:00:05 < 00:00:01

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