Title page for ETD etd-09072004-141252


Type of Document Dissertation
Author Contreras Rojas, Andrea Paz
URN etd-09072004-141252
Title Brucella abortus RB51 vaccine: Testing its Spectrum of Protective and Curative Characteristics
Degree PhD
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Schurig, Gerhardt G. Committee Chair
Ahmed, S. Ansar Committee Member
Boyle, Stephen M. Committee Member
Falkinham, Joseph O. III Committee Member
Sriranganathan, Nammalwar Committee Member
Vemulapalli, Ramesh Committee Member
Keywords
  • vaccine
  • therapeutic
  • RB51
  • Brucella
  • overexpression
  • cytokine
  • curative
  • gamma irradiation
  • cell mediated immunity
  • recombinant
  • Mycobacterium
  • paratuberculosis
  • avium.
Date of Defense 2004-07-30
Availability unrestricted
Abstract
Brucella abortus (BA) are gram-negative, facultative intracellular bacteria that cause abortions in cattle and debilitating illness in humans. The US is now virtually free of bovine brucellosis, but the disease is endemic in wildlife. The official brucellosis vaccine in the US is strain RB51 (RB51). It elicits protective cell-mediated immunity (CMI) against BA infections.

Mycobacterium avium subspecies paratuberculosis (MAP) causes paratuberculosis in ruminants. It is a slow growing intracellular parasite that requires CMI for its control, belongs to the genus Mycobacterium, and is closely related to M. avium avium (MA).

Using RB51 as a vector that induces strong protective CMI may be useful to protect against MAP if it expresses MAP protective antigens. Therefore, MAP 85A and 35kDa proteins were expressed at low levels in RB51, and the immune responses elicited by these vaccines in BALB/c mice were evaluated. Strong anti-Brucella immunity was generated, but the anti-mycobacterial response was low. To evaluate protective efficacy, a BALB/c model using MA was developed. When mice were challenged with MA, protection was obtained in some experiments but was inconsistent. This may be due to the low expression of MAP antigens in RB51.

Another objective was to evaluate the effect of an ongoing Brucella-infection on the efficacy of RB51 vaccination, and whether vaccination of already infected animals could have a curative effect. Mice acutely or chronically infected with virulent BA, rapidly cleared the RB51 vaccine organisms, but there was no significant decrease in the number of virulent BA.

Brucella spp. have been developed as biological weapons, but there are no vaccines to protect humans. The development of a very attenuated protective vaccine is necessary to prevent human infections, as well as to protect wildlife. To generate such a vaccine, RB51 based vaccines were irradiated to render them non-replicative, but metabolically active. We demonstrated that in general, irradiated and non-irradiated RB51 vaccines remain protective at levels similar to those elicited by the live vaccines. Therefore, irradiation of strain RB51 is an effective means of attenuating the strain without affecting its protective characteristics, and could eventually be used as a vaccine for wildlife and humans.

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