Title page for ETD etd-09182008-063547
|Type of Document
||Jeffrey, Stuart C.
||Epidermal growth factor receptor in equine gastric stratified squamous mucosa :effect of progressive ulceration on receptor density
||Master of Science
||Veterinary Medical Sciences
|Murray, Michael J.
|Pfeiffer, Carl J.
|Robertson, John L.
- gastric ulcers
- epidermal growth factor receptor
|Date of Defense
The objective of the study reported here was to document the
distribution of epidermal growth factor receptor (EGFr) and
quantitate receptor density in normal as well as ulcerated
equine gastric squamous mucosa. Fifteen horses with
endoscopically normal stomachs were divided into three equal
groups. Group 1 was a normal control. A protocol that
alternated 24 hour periods of free-choice hay with 24 hours of
feed deprivation was utilized to induce squamous mucosal
gastric ulceration in Group 2 (48 hours total off-feed) and
Group 3 (96 hours total off-feed). Gastric tissue was
collected from 3 stomach locations at post-mortem examination
and an avidin-biotin immunoperoxidase technique was developed
to stain the formalin-fixed tissue for EGFr. A computerized
image analysis system was used to measure EGFr area and mean
intensity values at four sites within the epithelium from the
basal cell layers to the lumen in the ulcer/erosion margin,
erosion bed, and 10-14 mm distant from the lesion.
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