Type of Document	Dissertation
Author	Jucker, Markus Thomas
URN	etd-09202005-091034
Title	Relationship of plasmids in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum
Degree	PhD
Department	Microbiology
Advisory Committee	Advisor Name Title Falkinham, Joseph O. III Committee Chair Bates, Robert C. Committee Member Boyle, Stephen M. Committee Member Johnson, John L. Committee Member Lacy, George H. Committee Member
Keywords	Mycobacterium intracellulare Genetics. Mycobacterium diseases Epidemiology. Plasmids Genetics
Date of Defense	1991-12-05
Availability	restricted
Abstract Bacteria of the Mycobacterium avium, M. intracellulare, and M. scrofulaceum group (MAIS) are opportunistic human pathogens and widespread in the environment. The first objective of this study was to demonstrate that plasmids from clinical isolates are closely related to plasmids from environmental MAIS isolates. A 12.9 kb plasmid, pVT2, from a clinical M. avium isolate, MDl, was cloned and used a a DNA probe to examine the relationship of MAIS plasmids. The pVT2 probe hybridized with plasmids isolated from MAIS strains from the environment, from patients without AIDS with pulmonary infections, and from AIDS patients with disseminated MAIS infections. Similar results were seen with a second probe derived from pLR 7, a 15.3 kb plasmid from clinical M. intracellulare strain LR 113. The similarity of plasmids from environmental and clinical isolates supports the hypothesis that the environment is a source of MAIS infection in humans.
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