Title page for ETD etd-09202005-091034
|Type of Document
||Jucker, Markus Thomas
||Relationship of plasmids in Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum
|Falkinham, Joseph O. III
|Bates, Robert C.
|Boyle, Stephen M.
|Johnson, John L.
|Lacy, George H.
- Mycobacterium intracellulare Genetics.
- Mycobacterium diseases Epidemiology.
- Plasmids Genetics
|Date of Defense
Bacteria of the Mycobacterium avium, M. intracellulare, and M.
scrofulaceum group (MAIS) are opportunistic human pathogens and widespread
in the environment. The first objective of this study was to demonstrate that
plasmids from clinical isolates are closely related to plasmids from
environmental MAIS isolates. A 12.9 kb plasmid, pVT2, from a clinical M.
avium isolate, MDl, was cloned and used a a DNA probe to examine the
relationship of MAIS plasmids. The pVT2 probe hybridized with plasmids
isolated from MAIS strains from the environment, from patients without AIDS
with pulmonary infections, and from AIDS patients with disseminated MAIS
infections. Similar results were seen with a second probe derived from pLR 7,
a 15.3 kb plasmid from clinical M. intracellulare strain LR 113. The similarity
of plasmids from environmental and clinical isolates supports the hypothesis
that the environment is a source of MAIS infection in humans.
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