Title page for ETD etd-09222002-193552


Type of Document Master's Thesis
Author Sisco, Daniel
URN etd-09222002-193552
Title Adeno-associated virus rep78 expression in Arabidopsis thaliana
Degree Master of Science
Department Biology
Advisory Committee
Advisor Name Title
Lederman, Muriel L. Committee Chair
Rutherford, Charles L. Committee Member
Winkel, Brenda S. J. Committee Member
Keywords
  • plant transformation
  • Adeno-associated virus
Date of Defense 2002-09-06
Availability unrestricted
Abstract
Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  01title.pdf 11.65 Kb 00:00:03 00:00:01 00:00:01 < 00:00:01 < 00:00:01
  02abstract.pdf 20.16 Kb 00:00:05 00:00:02 00:00:02 00:00:01 < 00:00:01
  03tableofcontents.pdf 21.21 Kb 00:00:05 00:00:03 00:00:02 00:00:01 < 00:00:01
  04intro.pdf 347.52 Kb 00:01:36 00:00:49 00:00:43 00:00:21 00:00:01
  05materialsandmethods.pdf 334.00 Kb 00:01:32 00:00:47 00:00:41 00:00:20 00:00:01
  06resultsanddiscussion.pdf 477.42 Kb 00:02:12 00:01:08 00:00:59 00:00:29 00:00:02
  07references.pdf 29.30 Kb 00:00:08 00:00:04 00:00:03 00:00:01 < 00:00:01
  08resume.pdf 15.05 Kb 00:00:04 00:00:02 00:00:01 < 00:00:01 < 00:00:01

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.