| Type of Document |
Master's Thesis |
| Author |
Sisco, Daniel
|
| URN |
etd-09222002-193552 |
| Title |
Adeno-associated virus rep78 expression in Arabidopsis thaliana |
| Degree |
Master of Science |
| Department |
Biology |
| Advisory Committee |
| Advisor Name |
Title |
| Lederman, Muriel L. |
Committee Chair |
| Rutherford, Charles L. |
Committee Member |
| Winkel, Brenda S. J. |
Committee Member |
|
| Keywords |
- plant transformation
- Adeno-associated virus
|
| Date of Defense |
2002-09-06 |
| Availability |
unrestricted |
Abstract
Adeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.
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02abstract.pdf |
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04intro.pdf |
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05materialsandmethods.pdf |
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06resultsanddiscussion.pdf |
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