Title page for ETD etd-10022007-144727
|Type of Document
||Kellman, Maxine Franchestcé
||Development of an antigen-specific ELISPOT to detect intestinal antibody responses to the swine whipworm, Trichuris suis
||Veterinary Medical Sciences
|Zajac, Anne M.
|Ahmed, S. Ansar
|Mansfield, Linda S.
|Schurig, Gerhardt G.
|Urban, J. F.
- Trichuris suis
|Date of Defense
The swine whipworm, Trichuris suis, is a parasite present throughout the United
States and is of concern to the swine industry worldwide because it is very pathogenic
to growing pigs. The economic threat posed by T. suis and other intestinal parasite
infections has created a strong interest in the development of parasite vaccines for the
swine industry. Use of a vaccine either alone or with anthelmintics should reduce the
economic losses. However, before effective parasite vaccines can be created, the swine
gastrointestinal immune response to parasite antigens must be understood. In this
study, an enzyme-linked immunospot (ELISPOT) assay was developed to measure total
and antigen-specific IgG and IgA antibody secreting cells (ASC) from gut-associated
lymphoid tissues (GALT) [mesenteric lymph node explants from jejunal region of small
intestine (SI-MLN) and cecum in large intestine (C-MLN); and ileocecal Peyer's
patches (IC-PP)] and lamina propria from the proximal colon removed from T. suis
infected pigs. Tbe local antibody responses were compared to peripheral antibody
responses found in the spleen and submandibular lymph nodes. The hypotheses to be
tested was that parasite antigen-specific antibody secreting cells would be greatest in
lymphoid tissue draining the site of infection compared to peripberal lymphoid tissues
and that 19A ASC would predominate over IgG ASC in the lamina propria of T. suis
infected pigs. The total IgG and IgA ASC frequencies for the spleen, SI-MLN, and ICPP
did not significantly change (P> 0.05) over time. For C-MLN, there was a
significant increase (p< 0.05) of total IgG ASC during a primary infection with T. suis.
Antigen-specific IgG ASC were greatest at the GALT site closest to the infection, CMLN,
whereas, antigen-specific IgA ASC predominated in the proximal colonic: lamina
propria. Host protection to T. suis develops after anthelmintic: treatment of a primary
exposure to parasite. The ELISPOT assay provided valuable information on the
localization and compartmentalization of the swine gastrointestinal immune response
to T. suis which resides in the cecum and proximal colon. In the future, this technique
may be useful for monitoring gastrointestinal immune parameters of pigs exposed to
a T. sllis vaccine.
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