Title page for ETD etd-10102005-131608


Type of Document Dissertation
Author Ankrapp, David P.
URN etd-10102005-131608
Title The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro
Degree PhD
Department Biochemistry and Nutrition
Advisory Committee
Advisor Name Title
Bevan, David R. Committee Chair
Ebel, Richard E. Committee Member
Potts, Malcolm Committee Member
Sitz, Thomas O. Committee Member
Veit, Hugo P. Committee Member
Keywords
  • Cancer cells Growth
  • Lungs Cancer
  • Somatomedin
Date of Defense 1993-05-05
Availability unrestricted
Abstract

The concentration of insulin-like growth factor I (IGF-I) in tissue taken from human non-small cell lung carcinoma (NSCLC) is 1.4- to 7-fold higher than the concentration of IGF-I in the surrounding normal lung tissue and therefore IGF-I may be involved in the growth of NSCLC. In this study it was determined that NSCLC cell lines (A549, A427, SK-LU-1) expressed the type I IGF-I receptor protein and IGF-I stimulated the proliferation of low density plated (2000 cells/cm2 growth area) carcinoma cells by 1.6- to 3- fold above control after a four day inCUbation period under serum-free conditions (A549, A427) or in the presence of 0.25% serum (SK-LU-1). In addition, when added to detergent-solubilized type I IGF receptors from A549 cells, IGF-I stimulated [1] a dose-dependent increase in the autophosphorylation of the type I IGF receptor, and [2] a dosedependent increase (1.5- to 4-fold) in the phosphorylation of a tyrosine kinase-specific substrate. These results suggest that the growth promoting activity of IGF-I for the lung carcinoma cells was mediated through the activation of the type I IGF receptor.

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