Title page for ETD etd-10102005-131608
|Type of Document
||Ankrapp, David P.
||The effect of insulin-like growth factor-I (IGF-I), and an IGF-I-like factor secreted by human lung fibroblasts, on the growth of human lung carcinoma cells in vitro
||Biochemistry and Nutrition
|Bevan, David R.
|Ebel, Richard E.
|Sitz, Thomas O.
|Veit, Hugo P.
- Cancer cells Growth
- Lungs Cancer
|Date of Defense
The concentration of insulin-like growth factor I
(IGF-I) in tissue taken from human non-small cell lung
carcinoma (NSCLC) is 1.4- to 7-fold higher than the
concentration of IGF-I in the surrounding normal lung tissue
and therefore IGF-I may be involved in the growth of NSCLC.
In this study it was determined that NSCLC cell lines (A549,
A427, SK-LU-1) expressed the type I IGF-I receptor protein
and IGF-I stimulated the proliferation of low density plated
(2000 cells/cm2 growth area) carcinoma cells by 1.6- to 3-
fold above control after a four day inCUbation period under
serum-free conditions (A549, A427) or in the presence of
0.25% serum (SK-LU-1). In addition, when added to detergent-solubilized
type I IGF receptors from A549 cells, IGF-I
stimulated  a dose-dependent increase in the autophosphorylation
of the type I IGF receptor, and  a dosedependent
increase (1.5- to 4-fold) in the phosphorylation
of a tyrosine kinase-specific substrate. These results
suggest that the growth promoting activity of IGF-I for the
lung carcinoma cells was mediated through the activation of the type I IGF receptor.
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