Title page for ETD etd-10142005-135755


Type of Document Dissertation
Author Ji, Wan
URN etd-10142005-135755
Title Expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicides
Degree PhD
Department Plant Physiology
Advisory Committee
Advisor Name Title
Kushad, Mosbah M. Committee Chair
Cramer, Carole L. Committee Member
Hatzios, Kriton K. Committee Member
Hess, John L. Committee Member
Orcutt, David M. Committee Member
Keywords
  • Corn Research
Date of Defense 1991-05-05
Availability restricted
Abstract

The activity of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, EC 1.1.1.34) is highly expressed in 4-day-old etiolated seedlings of normal ('DeKalb XL72AA'), dwarf (d5), and albino (7w3) maize (Zea mays L.). HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m2/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from 'Dekalb XL72AA' maize seedlings.

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