Title page for ETD etd-10192005-113259
|Type of Document
||Austin, T. Denise
||Characterization of the glpD and glpEGR operons of Escherichia coli k-12
||Biochemistry and Nutrition
|Larson, Timothy J.
|Dean, Dennis R.
|Gregory, Eugene M.
|Sitz, Thomas O.
- Escherichia coli research
- Phospholipids research.
|Date of Defense
The proteins required for catabolism of glycerol 3-
phosphate are encoded by the genes of the glp regulon of
Escherichia coli and are under negative transcriptional
regulation by the glpR-encoded repressor. The glpR gene is
adjacent to, and is transcribed divergently from, glpD.
GlpR and glpD are separated by two open reading frames,
designated glpE and glpG, encoding proteins of unknown
function. The glpD-encoded aerobic sn-glycerol 3-phosphate
dehydrogenase is a cytosplasmic membrane-associated
respiratory enzyme. The nucleotide sequence of glpD was
determined. An open reading frame of 501 codons was
preceded by a consensus Shine-Dalgarno sequence. The
proposed translational start and reading frame of glpD were
confirmed by determining the nucleotide sequence
across the fusion joint of a glpD-lacZ- translational fusion.
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