Title page for ETD etd-10202005-102838


Type of Document Dissertation
Author Liu, Zheng-Xian
URN etd-10202005-102838
Title Antioxidant activity of Mn-salophen complex and its effects on antioxidant enzymes in Escherichia coli
Degree PhD
Department Biochemistry and Anaerobic Microbiology
Advisory Committee
Advisor Name Title
Gregory, Eugene M. Committee Chair
Bevan, David R. Committee Member
Bunce, George Edwin Committee Member
Hess, John L. Committee Member
Misra, Hara P. Committee Member
Keywords
  • Superoxide dismutase
  • Escherichia coli Physiology
  • Antioxidants
Date of Defense 1994-11-05
Availability restricted
Abstract

Mn-salophen complex with superoxide-scavenging activity was prepared from manganese(III) acetate dihydrate and salophen in ethanol. Visible absorption spectrum of the red-brown solution exhibited a broad absorption band at 430 - 450 nm with two shoulders between 500 and 600 nm which were absent with either salophen or manganic acetate alone. Titration of salophen with manganese(III) was consistent with a 1:1 Mn to salophen stoichiometry of the complex based on changes in the absorbance at 500 nm or of superoxide scavenging activity. The SOD-like activity of the complex in the xanthine-xanthine oxidase/cytochrome c assay was 1450 units/mg salophen. The SOD activity of the complex was suppressed 50% in the presence of EDTA (1 mM), but was not altered in the presence of bovine serum albumin (1 mg/ml) or crude protein extract of E. coli QC779 sodA sodB (1 mg/ml). E. coli QC779 sodA sodB grew scantily after an 8 hour lag phase in aerobic M63 glucose minimal medium.

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