Title page for ETD etd-10202005-102838
|Type of Document
||Antioxidant activity of Mn-salophen complex and its effects on antioxidant enzymes in Escherichia coli
||Biochemistry and Anaerobic Microbiology
|Gregory, Eugene M.
|Bevan, David R.
|Bunce, George Edwin
|Hess, John L.
|Misra, Hara P.
- Superoxide dismutase
- Escherichia coli Physiology
|Date of Defense
Mn-salophen complex with superoxide-scavenging activity was prepared from
manganese(III) acetate dihydrate and salophen in ethanol. Visible absorption spectrum
of the red-brown solution exhibited a broad absorption band at 430 - 450 nm with two
shoulders between 500 and 600 nm which were absent with either salophen or
manganic acetate alone. Titration of salophen with manganese(III) was consistent with
a 1:1 Mn to salophen stoichiometry of the complex based on changes in the
absorbance at 500 nm or of superoxide scavenging activity. The SOD-like activity of
the complex in the xanthine-xanthine oxidase/cytochrome c assay was 1450 units/mg
salophen. The SOD activity of the complex was suppressed 50% in the presence of
EDTA (1 mM), but was not altered in the presence of bovine serum albumin (1 mg/ml) or crude protein extract of E. coli QC779 sodA sodB (1 mg/ml). E. coli
QC779 sodA sodB grew scantily after an 8 hour lag phase in aerobic M63 glucose
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