Title page for ETD etd-10312009-020219
|Type of Document
||Amanna, Ashwin E.
||Optical method of recording electrical activity in isolated rabbit hearts
||Master of Science
|Reed, Jeffrey Hugh
|Date of Defense
A recently developed optical method utilizes a single, implantable, optical fiber to
record electrical activity from isolated hearts stained with voltage-sensitive dyes. This
optical technique generates recordings of transmembrane potential from excitable
myocardial tissue, and remain free from stimulus artifacts that accompany
electro stimulation and hinder all standard electrode recording methods during the
application of high-voltage electrical shocks. The fiber optic system uses a l00J,lm
diameter core fiber which can record from epicardial surface, internal mid-myocardium,
or endocardial surfaces. The stained tissue is excited through the fiber and the resulting
fluorescence is transmitted through the same fiber to a photomultiplier tube. Changes in
fluorescence accompanying normal cardiac action potentials usually range from 1-5%.
Substantive motion related signals accompany normal beating hearts, drowning out the
actual signal that corresponds to change in membrane potential. When added to the
nutrient solution of the heart, an excitation / contraction decoupler restricts motion and
reduces the motion related signal making it easier to isolate the true membrane potential
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