Title page for ETD etd-10623859701581


Type of Document Master's Thesis
Author Flora, Jonathan P.
Author's Email Address jflora@juno.com
URN etd-10623859701581
Title The Effects of Temperature On The Durability Of Resistance Of Soybean To Soybean Mosaic Virus
Degree Master of Science
Department Plant Pathology, Physiology, and Weed Science
Advisory Committee
Advisor Name Title
Buss, Glenn R.
Warren, Herman L.
Tolin, Sue A. Committee Chair
Keywords
  • soybean mosaic virus
  • resistance
  • heat shock
Date of Defense 1997-05-08
Availability unrestricted
Abstract
The objectives of this study were to

determine the effects the temperature

sensitivity of alleles of Rsv1 in soybean

(Glycine max (L.) Merr.). Soybean cultivars

carrying alleles of Rsv1 were exposed to

several heat treatments designed to induce

heat shock protein production prior to

inoculation with soybean mosaic virus

(SMV). The heat treatment methods were

similar to those employed in the research with

N gene-tobacco mosaic virus studies. The

soybean cultivars used were Lee 69, York,

Kwanggyo, Ogden and PI96983, carrying

the Rsv, Rsv1-y, Rsv1-k, Rsv1-t, and Rsv1

allles of Rsv1, respectively, and were

selected to provide a range of reactions to

selected SMV pathotype groups. For

example Rsv1-y and Rsv1-k give a necrotic

response to SMV G4 and SMV G6,

respectively, while both are resistant to SMV

G1. To determine the durability of resistance

under heat shock conditions, the symptoms

were observed for changes in the phenotype

of the resistance response. Immunological

techniques were employed to determine the

vascular movement and localization of the

viral antigen in the plant. Heat treatments

used were found to induce HSP but to have

no effect on the resistance phenotype. A

detached leaf assay was used to test the

same Rsv1 alleles at constant high

temperatures. Primary trifoliolate leaflets

were removed and inoculated, then placed

into a continuously lighted incubator at 20 C

or 30 C. Leaf immunoprint assays were used

to determine the localization of the viral

antigen. The visible symptoms for necrotic

lesions and veins were observed for necrotic

phenotype-pathotype combinations but

mosaic symptoms were not observed on

detached leaves, as expected for inoculated

leaves. The detached leaf assay confirmed

that no change from the expected resistance

response of the Rsv1 alleles occurred at 30

C. A breakdown of resistance to SMV at

high temperature had been reported in

soybean by Tu and Buzzell (1987). The

resistance gene in which the high temperature

breakdown occurred has been determined to

be in Rsv3. Using cultivars and breeding lines

carrying Rsv3 in a similar experiment was

attempted in growth chambers. Preliminary

results suggest that Rsv3 is temperature

sensitive.

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