Title page for ETD etd-110798-012740


Type of Document Master's Thesis
Author Koechlein, David Jacob
Author's Email Address mule@vt.edu
URN etd-110798-012740
Title A STUDY OF THE COCCOID BODIES OF PROLINOBOBORUS FASCICULUS (AQUASPIRILLUM FASCICULUS)
Degree Master of Science
Department Biology
Advisory Committee
Advisor Name Title
Krieg, Noel R. Committee Chair
Claus, G. William Committee Member
Yousten, Allan A. Committee Member
Keywords
  • RNA
  • acridine orange
  • Viable but nonculturable
  • coccoid bodies
  • VBNC
Date of Defense 1998-09-17
Availability unrestricted
Abstract
Abstract: Following active growth, the aquatic gram-negative rod Prolinoborus fasciculus (Aquaspirillum fasciculus) exhibited a mass conversion from its culturable rod form to a nonculturable coccoid form. Chloramphenicol did not prevent the conversion. Attempts to obtain variants that would not convert to the coccoid form were unsuccessful. Although the coccoid form fluoresced with acridine orange, agarose gel electrophoresis revealed extensive ribosomal RNA degradation. Poly-ß-hydroxybutyrate, abundant in the vegetative rods, was not detectable in the coccoid cells. The results suggest that the coccoid form of A. fasciculus is a degenerative form rather than part of a life cycle.
Files
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  vitae.PDF 9.56 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01

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