Amino acids were determined in two types of chinchilla fur (chewed and normal), using three methods for analyses The methods
included microbiological assays of Barton-Wright(5) and two column
chromatography techniques of Moore and Stein (7, 8).
The data obtained from this investigation indicate that of the
16 amino acids analyzed, only one (lysine) was forum to be significantly
lower in the chewed fur, as indicated by the "t" test. This
difference between the normal and chewed fur was significant at the
2.5% 1evel. Also, arginine was lower and histidine was higher in the
chewed fur than in the normal fur, but these difference: were
not as conspicuous.
In general, the microbioligical analyses were more time consuming
end less reproducible than the chromatographic analysis. With the
exception of proline, glycine, and leucine, higher values were obtained
fer the mine acids using the microbiological methods than with the
column fractions. The greatest difficulty was encountered in analyzing
the basic amino acids.
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