Title page for ETD etd-11152013-040323


Type of Document Master's Thesis
Author Gore, Mitchell Taylor
URN etd-11152013-040323
Title Invitro protein synthesis and degradation and cathepsin D activity in the muscles of selenium-vitamin E deficient sheep
Degree Master of Science
Department Animal Science
Advisory Committee
Advisor Name Title
F. D. McCarthy Committee Chair
D. R. Notter Committee Member
K. E. Webb, Jr. Committee Member
S. H. Umberger Committee Member
Keywords
  • Muscle proteins
Date of Defense 1985-08-05
Availability restricted
Abstract
A study was conducted with 21 Suffolk x (Dorset—Coop-worth) lambs to investigate the in vitro synthesis and degradation

capabilities and the involvement of lysosomal enzymes

in Se-E deficient ovine skeletal muscle. A randomized

block design was utilized in which the lambs were subjected to 3 treatments: 1) A basal NaOH treated 85% corn, 15% hay diet to which was added a Se-E deficient mineral mixture (-Se—E), 2) The basal diet and deficient mineral mixture as fed in treatment 1 with the addition of a casein encapulated fat supplement fed at 3g•kg-1 body weight (-Se-E+fat), and

3) The basal diet plus a Se-E supplemented mineral mixture (+Se+E). Three lambs were killed at the start of the trial

with the remainder of the lambs killed after being on the diets an average of 110 days. In vitro protein synthesis and degradation was measured on the semitendinosus and triceps

brachi muscles. The activity of the lysosmal enzyme cathepsin D was estimated in skeletal and heart muscle. The skeletal, cardiac, kidney and liver tissues were analyzed for Se content. In addition, plasma E and whole blood Se levels were determined.

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