Title page for ETD etd-11152013-040457


Type of Document Master's Thesis
Author Bruns, Nicholas Joseph
URN etd-11152013-040457
Title Vitamin A deficiency, serum cortisol and immunoglobulin G levels in lambs
Degree Master of Science
Department Animal Science
Advisory Committee
Advisor Name Title
K. E. Webb, Jr. Committee Chair
E. T. Kornegay Committee Member
F. D. McCarthy Committee Member
Klaus D. Elgert Committee Member
Keywords
  • Hydrocortisone
Date of Defense 1986-04-05
Availability restricted
Abstract
Serum cortisol and immunoglobulin G (IgG>

concentrations were measured to investigate the

relationship between vitamin A status and immune function in lambs. Twenty-four crossbred ewe lambs were each fed 900 g·d-1 of a carotene—deficient diet composed of 95.5% whole

oats, 3% molasses, .5% trace mineral salt and 1% limestone. All lambs were injected monthly with vitamins D and E and

with selenium. The 12 control lambs also received a 100,000 IU oral dose of vitamin A palmitate in capsule form every 2

wk. All lambs were challenged by injecting them with 1 mg ovalbumin in 1 ml of Freund’s complete adjuvant. At the time of challenge, serum vitamin A levels for the control and A-deficient (A—def) lambs were 33.3 and 3.1 ug·dl-1

respectively. Blood was collected prior to and 6, 13, 20 and 34 d post—challenge. The lambs were then re—challenged using the same antigen and blood was obtained 1, 2, 6 and 22 d post—challenge. Lambs were sacrificed at the end of the second challenge period. Spleen weights were obtained and gross post—mortem observations were made at this time.

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