Title page for ETD etd-11192002-160104


Type of Document Master's Thesis
Author Vits, Lucia Carolina
Author's Email Address lvits@vt.edu
URN etd-11192002-160104
Title Biochemical Characterization of Normal Navicular Bone Flexor Surface Cartilage
Degree Master of Science
Department Veterinary Medical Sciences
Advisory Committee
Advisor Name Title
Pleasant, Robert Scott Committee Chair
Howard, Rick Dale Committee Member
White, Nathaniel A. II Committee Member
Keywords
  • biochemical
  • navicular
  • cartilage
Date of Defense 2002-11-12
Availability unrestricted
Abstract
Cartilage tissue specimens were obtained from the flexor surface of the navicular bone and distal radiocarpal bone articular surface (controls) from 8 horses 2 to 5 years old. Water, DNA, total collagen, total glycosaminoglycans, chondroitin sulphate, and keratan sulphate contents were determined. The results from each site were compared and the differences were analyzed by paired t-test (P < 0.05).

Significant differences were determined between the water content of the navicular bone flexor surface cartilage (68.32± 3.46 % ) and the distal radiocarpal bone articular surface cartilage (60.60± 4.09%). The total DNA content, total glycosaminoglycan content, total chondroitin sulphate content, and total keratan sulphate for the flexor surface of the navicular bone was: 524.51± 92.89 ng, 0.1533± 0.0338 mg, 0.1018± 0.0197 mg 0.0800± 0.0176 mg, and 0.0092± 0.0037 mg per mg of dry weight cartilage, respectively. The total DNA content, total glycosaminoglycan content, total chondroitin sulphate content, and total keratan sulphate for the distal radiocarpal articular surface cartilage was: 508.80± 70.16 ng, 0.1686± 0.00838 mg, 0.0919± 0.0191, 0.0615± 0.0109 mg, and 0.0074± 0.0029 mg per mg dry weight cartilage, respectively. Not significant differences were determined between these values.

We concluded that the cartilage of the flexor surface of the navicular bone is biochemically similar to hyaline articular cartilage, but differs from previous descriptions of fibrocartilage. Further studies are needed to determine types and proportions of collagen types of the flexor surface of the normal navicular bone. These findings establish a basis of comparison to assess navicular cartilage in aging, disease, and repair.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  lvits_thesis_final_rev3.pdf 1.37 Mb 00:06:20 00:03:15 00:02:51 00:01:25 00:00:07

Browse All Available ETDs by ( Author | Department )

dla home
etds imagebase journals news ereserve special collections
virgnia tech home contact dla university libraries

If you have questions or technical problems, please Contact DLA.