Title page for ETD etd-11302007-115548


Type of Document Dissertation
Author Cubitt, Tania Anne
URN etd-11302007-115548
Title Long Term and Short Term Changes in Leptin, Insulin and Glucose in Grazing Thoroughbred Mares
Degree PhD
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
Geor, Raymond J. Committee Chair
Bowen, John M. Committee Member
Gwazdauskas, Francis C. Committee Member
Kronfeld, David S. Committee Member
Splan, Rebecca K. Committee Member
Staniar, William Burton Committee Member
Keywords
  • leptin
  • glucose
  • insulin
  • estrous cycle
  • follicle
  • season
  • circadian pattern
Date of Defense 2007-11-05
Availability unrestricted
Abstract
The objective was to evaluate the insulin-leptin interactions in grazing mares and their impact on aspects of reproductive function using a series of four studies. Fourteen mares were first examined, 10 grazing and 4 confined to stalls in four 22-h studies in April, August, October (2005) and January (2006). There was a positive relationship between insulin and leptin concentrations (r = 0.50; P < 0.001). The second study used 24 mares maintained at pasture and fed supplements rich in either FF, or SS, or pasture forage. Nonstructural carbohydrate (NSC) content of forage was correlated to insulin concentration (r = 0.55; P < 0.01). Insulin was associated with leptin (r = 0.55; P < 0.001) and progesterone (r = 0.48; P < 0.001). In the third study nine mares adapted to FF, SS or forage only underwent two FSIGT tests; once during the luteal phase and again during the follicular phase of the estrous cycle. Minimal model analysis was used to describe insulin sensitivity (SI), glucose effectiveness (Sg), acute insulin response to glucose (AIRg) and the disposition index (DI). SI was lower (P < 0.001) in the luteal phase (3.1 ± 0.6) compared to the follicular phase (5.0 ± 0.6). In the fourth study 15 mares had their follicular fluid collected during the luteal and follicular phase of the estrous cycle. Insulin concentration (ln (x+1)) was 52% higher (P < 0.01) in large (> 25 mm) follicles (1.4 ± 0.1 mIU/L) than either medium (16 to 25 mm) or small (le; 15 mm) follicles (0.9 ± 0.1; 0.9 ± 0.1 mIU/L, respectively) irrespective of estrous cycle phase. A correlation was observed between follicular fluid (FFL) leptin and plasma leptin (r = 0.30; P < 0.001). A similar relationship was observed between FFL insulin and plasma insulin (r = 0.25; P < 0.001). Plasma insulin and leptin were positively associated (r = 0.45, P < 0.0001), along with FFL insulin and FFL leptin (r = 0.46, P < 0.0001). Both leptin and insulin were affected by season and this observation contradicts the use of single sample analysis for determining detrimental concentrations of these hormones.
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