Title page for ETD etd-12162009-020346

Type of Document

Master's Thesis

Author

Gibbons, John R.

URN

etd-12162009-020346

Title

Ultrasound-guided transvaginal follicular aspiration to provide a source of bovine oocytes for gene microinjection

Degree

Master of Science

Department

Dairy Science

Advisory Committee

Advisor Name	Title
Gwazdauskas, Francis C.	Committee Chair
Beal, W. E.	Committee Member
Pearson, Ronald E.	Committee Member
Vinson, William E.	Committee Member

Keywords

Ovum.

Date of Defense

1994-05-15

Availability

restricted

Abstract

Three experiments were conducted to determine an efficient method of obtaining oocytes from cows via ultrasound-guided transvaginal follicular aspiration. Transvaginal oocyte recovery combined with in vitro maturation, fertilization, and culture (IVMlIVFIIVC) can produce pre-implantation stage bovine embryos and also supply a source of ova for gene microinjection. In Experiment 1, once- (IX) vs. twice-weekly (2X) oocyte recovery frequencies were compared. No differences in recovery per session were observed (IX = 6.8 vs. 2X = 6.3 oocytes/session; p>O.1 0). However, the 2X group generated more oocytes on a weekly basis (IX = 6.8 vs. 2X = 12.6 oocytes/week; pO.IO) In these experiments, twice-weekly follicular aspiration without exogenous FSH, was the best scheme of oocyte recovery. This program provided a source of consistent, high quality oocytes that responded favorable to the IVMIIVFIIVC system and microinjection.

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