Title page for ETD etd-12172002-182647


Type of Document Master's Thesis
Author Morris, Bruce C.
URN etd-12172002-182647
Title Intestinal Mucosal Mast Cell Immune Response and Pathogenesis of Two Eimeria Acervulina Isolates in Broiler Chickens
Degree Master of Science
Department Animal and Poultry Sciences
Advisory Committee
Advisor Name Title
McElroy, Audrey P. Committee Chair
Caldwell, David J. Committee Member
Danforth, Harry D. Committee Member
Pierson, Frank William Committee Member
Keywords
  • immunovariability
  • broilers
  • immunity
  • Eimeria
  • mast cell
Date of Defense 2002-12-05
Availability unrestricted
Abstract
Five experiments were conducted comparing differential intestinal immune responses to two isolates of Eimeria acervulina (EA), EA1 and EA2. In three experiments, broiler chicks were divided into control (non-challenged), EA1, or EA2 challenged (14 days of age) groups. On day 6 post-challenge (PC), changes in body weight were determined, intestinal lesions were scored, and duodenal tissue was evaluated for morphometric alterations and mucosal mast cell responses. EA1 produced duodenal lesions and reduced villus height to crypt depth ratios when compared to controls; however, no differences were found in mast cell counts. EA2 produced differing results, and observed data were suggestive of an intestinal secretory response when compared to EA1 or controls. In Experiment 4, tissues were analyzed from day 2 through day 6 PC. Villus atrophy and crypt hyperplasia were heightened on day 5 PC in both challenged groups. Mast cell counts were significantly greater on days 3 and 4 PC in EA1 birds. In Experiment 5, EA2 oocysts were cleaned with 5.25% sodium hypochlorite to evaluate the possibility of a bacterial contaminant contributing to the pathogenesis of intestinal alterations. Weight gains were decreased by challenge and villus heights and crypt depths were significantly altered in challenged birds, resulting in lower villus to crypt ratios, however, there were no differences in mast cell number. These data are indicative of differential host response and immunovariability between different isolates of the same Eimeria species and are suggestive of mast cell involvement in coccidial immunity in broiler chickens.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Table1.pdf 8.50 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01
  Table2.pdf 9.82 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01
  Table3.pdf 10.28 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01
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  Table6.pdf 8.98 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01
  Thesis.pdf 431.69 Kb 00:01:59 00:01:01 00:00:53 00:00:26 00:00:02
  Title.pdf 19.49 Kb 00:00:05 00:00:02 00:00:02 00:00:01 < 00:00:01

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